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Citations & References (48)
Invitrogen™
Carboxy-DCFDA (5-(and-6)-Carboxy-2',7'-Dichlorofluorescein Diacetate), mixed isomers
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| C369 | 100 mg |
製品番号（カタログ番号） C369
価格（JPY）
73,900
Each
数量:
100 mg
Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.
To make a concentrated stock solution from 1–10 mM, dissolve in either high quality anhydrous DMSO, DMF, or 100% ethanol. Once prepared, use the stock solutions within a short time period.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
検出法蛍光
染色剤タイプpHインジケータ
分子量529.29
数量100 mg
出荷条件室温
使用対象（アプリケーション）Polar Tracer, pH Indicator
使用対象 (装置)Fluorescence Microscope
製品タイプCarboxy-DCFDA
Unit SizeEach
組成および保存条件
Store in freezer (-5°C to -30°C).
引用および参考文献 (48)
引用および参考文献
Abstract
Mitochondrial production of reactive oxygen species mediate dicumarol-induced cytotoxicity in cancer cells.
Journal:J Biol Chem
PubMed ID:17040906
'Dicumarol is a naturally occurring anticoagulant derived from coumarin that induces cytotoxicity and oxidative stress in human pancreatic cancer cells (Cullen, J. J., Hinkhouse, M. M., Grady, M., Gaut, A. W., Liu, J., Zhang, Y., Weydert, C. J. D., Domann, F. E., and Oberley, L. W. (2003) Cancer Res. 63,
Profilin 1 is essential for retention and metabolism of mouse hematopoietic stem cells in bone marrow.
Journal:
PubMed ID:24385538
'How stem cells interact with the microenvironment to regulate their cell fates and metabolism is largely unknown. Here we demonstrated that the deletion of the cytoskeleton-modulating protein profilin 1 (pfn1) in hematopoietic stem cell (HSCs) led to bone marrow failure, loss of quiescence, and mobilization and apoptosis of HSCs in
KEAP1 is a redox sensitive target that arbitrates the opposing radiosensitive effects of parthenolide in normal and cancer cells.
Journal:
PubMed ID:23674500
'Elevated oxidative stress is observed more frequently in cancer cells than in normal cells. It is therefore expected that additional exposure to a low level of reactive oxygen species (ROS) will push cancer cells toward death, whereas normal cells might maintain redox homeostasis through adaptive antioxidant responses. We previously showed
G-protein ligands inhibit in vitro reactions of vacuole inheritance.
Journal:J Cell Biol
PubMed ID:8027189
'During budding in Saccharomyces cerevisiae, maternal vacuole material is delivered into the growing daughter cell via tubular or vesicular structures. One of the late steps in vacuole inheritance is the fusion in the bud of vesicles derived from the maternal vacuole. This process has been reconstituted in vitro and requires
A trace amount of the human pro-apoptotic factor Bax induces bacterial death accompanied by damage of DNA.
Journal:J Biol Chem
PubMed ID:9556634
'An amount of human pro-apoptotic Bax as low as 0.01% of total protein was sufficient to cause cell death in Escherichia coli. The bacterial cell death was examined using a viable bacteria-specific fluorescence indicator system and loss of colony formation ability. Co-expression of anti-apoptotic Bcl-xL showed a modest inhibitory effect