What is the excitation and emission wavelength for rhodamine?
Rhodamine is a generic term for a wide variety of cationic dyes whose fluorescence emission can range from green, orange to red. The table below lists the excitation and emission maxima (nm), as well as molar extinction coefficients (EC; cm-1 M-1), for various rhodamine dyes (data derived with dye dissolved in methanol).
Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We designed molecular beacons to detect a point mutation in the methylenetetrahydrofolate reductase (MTHFR) gene, a mutation that has been related to an increased risk for cardiovascular disease and neural tube defects. The application of molecular beacons enables fast, semiautomated, ... More
Sites of tubulin polymerization in PC12 cells.
Authors:Keith CH, Blane K
Journal:J Neurochem
PubMed ID:2179472
'The site at which tubulin enters into polymer in the neuritic process is a very important datum in terms of our understanding of the mechanism of transport of the microtubular cytoskeleton out the axon. If the form of tubulin being transported out the axon is the microtubule, then assembly of ... More
Kinetic studies by fluorescence resonance energy transfer employing a double-labeled oligonucleotide: hybridization to the oligonucleotide complement and to single-stranded DNA.
Authors:Parkhurst KM, Parkhurst LJ
Journal:Biochemistry
PubMed ID:7819209
'A single 16-base oligodeoxyribonucleotide was labeled at the 3''-end with fluorescein and at the 5''-end with x-rhodamine (R*oligo*F); the chromophores served as a donor/acceptor pair, respectively, for Förster resonance energy transfer. We exploited the striking differences in the steady-state emission spectra of the R*oligo*F as a single strand and in ... More
Microtubules of the kinetochore fiber turn over in metaphase but not in anaphase.
Authors:Gorbsky GJ, Borisy GG
Journal:J Cell Biol
PubMed ID:2668301
'In previous work we injected mitotic cells with fluorescent tubulin and photobleached them to mark domains on the spindle microtubules. We concluded that chromosomes move poleward along kinetochore fiber microtubules that remain stationary with respect to the pole while depolymerizing at the kinetochore. In those experiments, bleached zones in anaphase ... More