Cascade Blue™ hydrazide, Trisodium Salt
Cascade Blue™ hydrazide, Trisodium Salt
Invitrogen™

Cascade Blue™ hydrazide, Trisodium Salt

細胞非透過性を持ち固定可能な極性トレーサー、Cascade Blue™ヒドラジドは、細胞-細胞融合および細胞-リポソーム融合のほか、膜透過性の調査、およびピノサイトーシス中のギャップ結合または細胞取り込みを介した輸送の調査に使用できます。この水溶性トレーサーは、全細胞パッチクランプ、イオン導入、飲小胞の浸透性溶解または同等の方法によって細胞に導入できます詳細を見る
製品番号(カタログ番号)数量
C68710 mg
製品番号(カタログ番号) C687
価格(JPY)
61,000
Each
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数量:
10 mg
細胞非透過性を持ち固定可能な極性トレーサー、Cascade Blue™ヒドラジドは、細胞-細胞融合および細胞-リポソーム融合のほか、膜透過性の調査、およびピノサイトーシス中のギャップ結合または細胞取り込みを介した輸送の調査に使用できます。この水溶性トレーサーは、全細胞パッチクランプ、イオン導入、飲小胞の浸透性溶解または同等の方法によって細胞に導入できます。また、タンパク質や他の水溶性バイオポリマーのカルボン酸は、EDAC(E2247)などの水溶性カルボジイミドを使用して水溶液中で、この分子と結合することができます。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
化学反応性カルボン酸、ケトン、アルデヒド
発光412 nm
励起399 nm
標識または色素Cascade Blue™
分子量596.44 G/MOL
製品タイプヒドラジド
数量10 mg
反応性部分アミン,ヒドラジド
出荷条件室温
標識タイプ従来の色素
製品ラインCASCADE BLUE
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。

よくあるご質問(FAQ)

What is the recommended concentration for preparation of Cascade Blue hydrazide, Trisodium Salt (Cat. No. C687) stock solution?

We recommend preparing a 1% w/v stock solution in sterile water. Please note that the dye can aggregate in solution over time.

引用および参考文献 (24)

引用および参考文献
Abstract
Parathyroid hormone controls the size of the intracellular Ca(2+) stores available to receptors linked to inositol trisphosphate formation.
Authors:Short AD, Taylor CW
Journal:J Biol Chem
PubMed ID:10636879
'In HEK 293 cells stably expressing type 1 parathyroid (PTH) receptors, PTH stimulated release of intracellular Ca(2+) stores in only 27% of cells, whereas 96% of cells responded to carbachol. However, in almost all cells PTH potentiated the response to carbachol by about 3-fold. Responses to carbachol did not desensitize, ... More
Neuropeptides exert direct effects on rat thymic epithelial cells in culture.
Authors:Head GM, Mentlein R, von Patay B, Downing JE, Kendall MD
Journal:Dev Immunol
PubMed ID:9716910
'To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue ... More
Oligosaccharide organization of the beta-subunits of pig kidney Na+/K+-ATPase.
Authors:Amler E, Staffolani R, Baranska J, Obsil T, Urbanová P, Bertoli E, Mazzanti L
Journal:Physiol Res
PubMed ID:9730046
'The distance between the beta-subunits of Na+/K+-ATPase isolated from pig dark red kidney medulla was determined by Förster energy transfer. First, oligosaccharides of the beta-subunit were shown to be labelled with three fluorophores: Lucifer yellow (LY), Lissamine rhodamine B sulfonyl hydrazine (LRSH) and Cascade blue (CB). Further, LY and LRSH ... More
Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine.
Authors:Geoghegan KF, Stroh JG
Journal:Bioconjug Chem
PubMed ID:1325188
'The 2-amino alcohol structure -CH(NH2)CH(OH)- exists in proteins and peptides in N-terminal Ser or Thr and in hydroxylysine. Its very rapid oxidation by periodate at pH 7 generates an aldehyde in the peptide and is the first step in a method for site-directed labeling with biotin or a fluorescent reporter. ... More
Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions.
Authors:Becker DL, Evans WH, Green CR, Warner A
Journal:J Cell Sci
PubMed ID:7615666
'Gap junctions allow direct communication between cells without recourse to the extracellular space and have been widely implicated as important mediators of cell-cell signalling. They are constructed from the connexin proteins, which form a large family, and individual connexins show complex spatial and temporal variations in their expression patterns. Understanding ... More