CellTracker™ CM-DiI Dye
CellTracker™ CM-DiI Dye
Citations & References (61)
Invitrogen™

CellTracker™ CM-DiI Dye

CellTracker™ CM-DiIは、細胞の移動や位置のモニタリングに最適な蛍光色素です。この色素は十分に保持されているため、細胞の動きを多世代で追跡できます。また、赤色励起 / 発光スペクトルは、緑色の蛍光色素およびタンパク質によるマルチプレックスに最適です詳細を見る
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製品番号（カタログ番号）数量
C70011mg
C700020 x 50 μg
製品番号（カタログ番号） C7001
価格（JPY）
75,700
Each
お問い合わせください ›
数量:
1mg
CellTracker™ CM-DiIは、細胞の移動や位置のモニタリングに最適な蛍光色素です。この色素は十分に保持されているため、細胞の動きを多世代で追跡できます。また、赤色励起 / 発光スペクトルは、緑色の蛍光色素およびタンパク質によるマルチプレックスに最適です。

異なる蛍光スペクトルや長いトラッキングが必要ですか？その他の哺乳類細胞トラッキング製品をご覧ください。

•培地の除去、色素の添加、30分間のインキュベート、および画像細胞は簡単に使用できます
•蛍光シグナル保持時間72時間超（通常3～6世代）
•赤色励起／発光スペクトル（最大553/570 nm）は多重化に最適
•低細胞毒性は、生存率や増殖に影響しません

CellTracker™ CM-DiI蛍光色素は、細胞膜を自由に通過して細胞に入り、細胞膜非透過性反応産物に変換されるように設計されています。CellTracker™ CM-DiI色素は、数世代にわたって生細胞に保持されます。色素はドーター細胞に転写されますが、集団内の隣接する細胞には転写されません。CellTracker™ CM-DiI色素は、蛍光を72時間以上表示するように設計されており、この色素は理想的なトラッキング特性を示します。安定しており、作業濃度では無毒で、細胞内に良好に保持され、生理学的pHでは明るい蛍光を示します。さらに、CellTracker™ CM-DiI色素の励起および発光スペクトルはGFP（緑色蛍光タンパク質）スペクトルから十分に分離されているため、マルチプレックスが可能です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
色黄色
検出法蛍光
使用対象 (装置)蛍光顕微鏡
製品ラインCellTracker
数量1mg
出荷条件室温
標識タイプ蛍光タンパク質
製品タイプ色素
SubCellular Localization細胞膜&脂質, Lipids
Unit SizeEach
組成および保存条件
フリーザー(-5°C∼-30°C)に保存し、遮光してください。

よくあるご質問（FAQ）

I want to do a cell migration study for around 4 hours and need to fluorescently label the cells with a dye. What do you recommend?

Calcein, AM and FDA (fluorescein diaceate) are examples of some dyes used for this application. Since these dyes are not incorporated or covalently attached to any cellular components, they may have a short retention time as some cell types may actively efflux the dye out of the cells. The CellTracker and CellTrace dyes include either a mild thiol-reactive chloromethyl group or amine-reactive succinnimidyl ester group to allow for covalent binding to cellular components, providing for better retention. As with any reagent, one should empirically determine retention times for the cell type used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C18 and the other cell line with green fluorescent DiO C18. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cells with Calcein, AM, but when I imaged the next day, there was no fluorescence from Calcein. Why?

Calcein, AM is a good choice for cell tracking and as a general cytoplasmic stain. However, it doesn't bind to anything and may be actively pumped out of the cells within a couple hours, which is likely what happened. The retention of Calcein within live cells is dependent upon the inherent properties of the cell type and culture conditions.

For long-term imaging, you may wish to consider a reactive cytoplasmic stains such as CFDA, SE or the CellTracker and CellTrace dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare a stock solution of CellTracker CM-DiI Dye (Cat. No. C7000, C7001)?

A stock solution of CellTracker CM-DiI Dye (Cat. No. C7000, C7001) may be prepared in dimethyl formamide (DMF), dimethylsulfoxide (DMSO), or ethanol at a concentration of 1-2 mg/mL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellTracker™ CM-DiI Dye, 1mg FAQs

引用および参考文献 (61)

引用および参考文献
Abstract
The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.
Authors:Andrade W, Seabrook TJ, Johnston MG, Hay JB
Journal:J Immunol Methods
PubMed ID:8765171
'In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most ... More
Characterization of antisera specific to NK1, NK2, and NK3 neurokinin receptors and their utilization to localize receptors in the rat gastrointestinal tract.
Authors:Grady EF, Baluk P, Böhm S, Gamp PD, Wong H, Payan DG, Ansel J, Portbury AL, Furness JB, McDonald DM, Bunnett NW
Journal:J Neurosci
PubMed ID:8824334
'Understanding the physiological role of tachykinins requires precise cellular and subcellular localization of their receptors. We raised antisera by immunizing rabbits with peptides corresponding to portions of the intracellular tails of the rat neurokinin 1, 2, and 3 receptors (NK1-R, NK2-R, NK3-R). Receptors were localized by immunofluorescence and confocal microscopy. ... More
Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes.
Authors:Anderson SA, Eisenstat DD, Shi L, Rubenstein JL
Journal:Science
PubMed ID:9334308
'Although previous analyses indicate that neocortical neurons originate from the cortical proliferative zone, evidence suggests that a subpopulation of neocortical interneurons originates within the subcortical telencephalon. For example, gamma-aminobutyric acid (GABA)-expressing cells migrate in vitro from the subcortical telencephalon into the neocortex. The number of GABA-expressing cells in neocortical slices ... More
Analysis of vertical fluorescence resonance energy transfer from the surface of a small-diameter sphere.
Authors:Jones GM, Wofsy C, Aurell C, Sklar LA
Journal:Biophys J
PubMed ID:9876165
'Fluorescence resonance energy transfer (FRET) measurements have been used to analyze fluorophore separations in a number of varying geometries, including small particles and extended surfaces. This study focuses on the geometry created by a donor extended above the surface of a small sphere (radius < R0), where the acceptors are ... More
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma.
Authors:Khakoo AY, Pati S, Anderson SA, Reid W, Elshal MF, Rovira II, Nguyen AT, Malide D, Combs CA, Hall G, Zhang J, Raffeld M, Rogers TB, Stetler-Stevenson W, Frank JA, Reitz M, Finkel T,
Journal:J Exp Med
PubMed ID:16636132
'Emerging evidence suggests that both human stem cells and mature stromal cells can play an important role in the development and growth of human malignancies. In contrast to these tumor-promoting properties, we observed that in an in vivo model of Kaposi''s sarcoma (KS), intravenously (i.v.) injected human mesenchymal stem cells ... More
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