CyQUANT™ Cell Proliferation Assays
CyQUANT™ Cell Proliferation Assays
Citations & References (65)
Invitrogen™

CyQUANT™ Cell Proliferation Assays

CyQUANT™ NF細胞増殖アッセイは、集団中の細胞をカウントし、マイクロプレートフォーマットで増殖を測定するための迅速かつ高感度な方法を提供します。CyQUANT™ NF細胞増殖アッセイの特長：•MTTやalamarBlue™アッセイよりも高感度• ウェルあたり100～20,000細胞の直線的検出範囲（96ウェルマイクロプレート）• アッセイは1時間で完了増殖を測定する迅速かつシンプルな方法CyQUANT™詳細を見る
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製品番号（カタログ番号）細胞タイプ数量
C7026For cells in culture1000 Assays
C35011Direct Cell10 Microplates
C35013Direct Red Cell10個のマイクロプレート
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 mL
製品番号（カタログ番号） C7026
価格（JPY）
90,000
Each
お問い合わせください ›
細胞タイプ:
For cells in culture
数量:
1000 Assays
CyQUANT™ NF細胞増殖アッセイは、集団中の細胞をカウントし、マイクロプレートフォーマットで増殖を測定するための迅速かつ高感度な方法を提供します。

CyQUANT™ NF細胞増殖アッセイの特長：

•MTTやalamarBlue™アッセイよりも高感度
• ウェルあたり100～20,000細胞の直線的検出範囲（96ウェルマイクロプレート）
• アッセイは1時間で完了

増殖を測定する迅速かつシンプルな方法
CyQUANT™ NF細胞増殖アッセイでは、細胞溶解、長時間のインキュベーション、放射能、細胞からの染色の除去は不要です。CyQUANT™ NFアッセイでは、細胞透過性のDNA結合色素を細胞膜透過処理試薬と組み合わせて使用することで、オリジナルのCyQUANT™細胞増殖アッセイの凍結融解ステップが不要になります。CyQUANT™ NF細胞増殖アッセイキットは、96ウェルまたは384ウェルのマイクロプレートフォーマットで使用でき、より小さなサンプルサイズ用の200アッセイキット（C35007）と、ハイスループットアプリケーション用の1000アッセイキット（C35006）の2種類の構成で使用できます。

alamarBlue™はTREK Diagnostic Systemsの登録商標です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞透過性Cell-impermeant
細胞タイプFor cells in culture
概要CyQUANT™ Cell Proliferation Assay, for cells in culture
検出法蛍光
染色剤タイプその他の標識または色素
励起／発光480/520
フォーマット96-ウェルプレート
数量1000 Assays
出荷条件室温
色Green
Emission520 nm
Excitation Wavelength Range480 nm
使用対象（アプリケーション）増殖アッセイ
使用対象 (装置)マイクロプレートリーダー
製品ラインCyQUANT
製品タイプ細胞増殖アッセイ
Unit SizeEach
組成および保存条件
CyQUANT™ GR色素、細胞溶解バッファー、およびバクテリオファージ λ DNAが含まれています。

よくあるご質問（FAQ）

Can CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026) be used on organoids in Matrigel medium?

CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) has been validated for use with the AlgiMatrix 3D Culture System, but we cannot guarantee that it will work with other 3D culture systems.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture (Cat. No. C7026)?

The composition of the cell lysis buffer in CyQUANT Cell Proliferation Assay, for cells in culture is proprietary.

Which cell proliferation assays can I use with 3D culture systems?

alamarBlue Cell Viability Reagent, PrestoBlue Cell Viability Reagent, and CyQUANT Cell Proliferation Assay Kit (Cat. No. C7026) have been validated for use with the AlgiMatrix 3D Culture System and should also work with other 3D culture systems. For further details, please refer to this poster (https://aacrjournals.org/cancerres/article/70/8_Supplement/3234/563757/Abstract-3234-AlgiMatrixT-3-D-cell-culture-system) and protocol (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/3-d-cell-culture-protocol/algimatrix-viability-using-cyquant.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer any products to measure neuronal cell health?

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (65)

引用および参考文献
Abstract
Growth factor-induced proliferation in corneal epithelial cells is mediated by 12(S)-HETE.
Authors:Ottino P,Taheri F,Bazan HE
Journal:Experimental eye research
PubMed ID:12697425
PURPOSE: Previous studies in our laboratory have shown that 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE), a product of 12-lipoxygenase (12-LOX) activity, is the predominant metabolite formed in rabbit corneas after injury. The present study was undertaken to investigate the effects of epidermal growth factor (EGF), hepatocyte growth factor (HGF), and keratinocyte growth factor ... More
Caspase activation contributes to delayed death of heat-stressed striatal neurons.
Authors:White MG, Emery M, Nonner D, Barrett JN
Journal:J Neurochem
PubMed ID:14622126
'Hyperthermia can contribute to brain damage both during development and post-natally. We used rat embryonic striatal neurons in culture to study mechanisms underlying hyperthermia-induced neuronal death. Heat stress at 43 degrees C for 2 h produced no obvious signs of damage during the first 12 h after the stress, but ... More
Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin.
Authors:Moser TL, Kenan DJ, Ashley TA, Roy JA, Goodman MD, Misra UK, Cheek DJ, Pizzo SV
Journal:Proc Natl Acad Sci U S A
PubMed ID:11381144
'Angiostatin blocks tumor angiogenesis in vivo, almost certainly through its demonstrated ability to block endothelial cell migration and proliferation. Although the mechanism of angiostatin action remains unknown, identification of F(1)-F(O) ATP synthase as the major angiostatin-binding site on the endothelial cell surface suggests that ATP metabolism may play a role ... More
Overexpression of Na(+)/K (+)-ATPase parallels the increase in sodium transport and potassium recycling in an in vitro model of proximal tubule cellular ageing.
Authors:Silva E, Gomes P, Soares-da-Silva P,
Journal:J Membr Biol
PubMed ID:17334838
'Na(+)/K(+)-ATPase plays a key role in the transport of Na(+) throughout the nephron, but ageing appears to be accompanied by changes in the regulation and localization of the pump. In the present study, we examined the effect of in vitro cell ageing on the transport of Na(+) and K(+) ions ... More
Identification of the binding site for fibrinogen recognition peptide gamma 383-395 within the alpha(M)I-domain of integrin alpha(M)beta2.
Authors:Yakubenko VP, Solovjov DA, Zhang L, Yee VC, Plow EF, Ugarova TP
Journal:J Biol Chem
PubMed ID:11278633
'The leukocyte integrin alpha(M)beta(2) (Mac-1, CD11b/CD18) is a cell surface adhesion receptor for fibrinogen. The interaction between fibrinogen and alpha(M)beta(2) mediates a range of adhesive reactions during the immune-inflammatory response. The sequence gamma(383)TMKIIPFNRLTIG(395), P2-C, within the gamma-module of the D-domain of fibrinogen, is a recognition site for alpha(M)beta(2) and alpha(X)beta(2). ... More
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