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Citations & References (18)
Invitrogen™
DQ™ Collagen, type IV From Human Placenta, Fluorescein Conjugate
蛍光性のあるDQ™コラーゲンは、コラーゲンの活性を直接モニタリングする目的で使用できます。DQ™基質は天然基質の類似体であり、蛍光シグナルがほとんど存在しなくなるように、過剰な数の蛍光色素が結合されています。このようなシグナルの消光効果は、インタクトな基質上の色素が近接していることが原因で発生します。酵素作用によって基質が加水分解されると色素が互いに分離し、蛍光シグナルが増加します。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| D12052 | 1 mg |
製品番号（カタログ番号） D12052
価格（JPY）お問い合わせください ›
78,800
Each
数量:
1 mg
蛍光性のあるDQ™コラーゲンは、コラーゲンの活性を直接モニタリングする目的で使用できます。DQ™基質は天然基質の類似体であり、蛍光シグナルがほとんど存在しなくなるように、過剰な数の蛍光色素が結合されています。このようなシグナルの消光効果は、インタクトな基質上の色素が近接していることが原因で発生します。酵素作用によって基質が加水分解されると色素が互いに分離し、蛍光シグナルが増加します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
製品ラインDQ
数量1 mg
出荷条件室温
基質プロテアーゼ基質
検出法蛍光
形状凍結乾燥
Substrate Propertiesタンパク質ベースの基質
Target Enzymeメタロプロテイナーゼ
Unit SizeEach
組成および保存条件
フリーザー（-5～-30度）に保存し、遮光してください。
引用および参考文献 (18)
引用および参考文献
Abstract
Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.
Journal:Exp Cell Res
PubMed ID:12581740
'Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using specific cathepsin
Matrix metalloproteinase activity in urine of patients with renal cell carcinoma leads to degradation of extracellular matrix proteins: possible use as a screening assay.
Journal:J Urol
PubMed ID:12629409
'PURPOSE: Localized renal cell carcinoma is usually curable by nephrectomy. However, a large fraction of patients already present with metastatic disease, which results in a poor outcome. Currently no clinically relevant screening assay is available to detect early stage renal cell carcinoma. We investigated whether urinary extracellular matrix (ECM) proteins
Imaging proteolysis by living human glioma cells.
Journal:Biol Chem
PubMed ID:11517931
'Degradation of basement membrane is an essential step for tumor invasion. In order to study degradation in real time as well as localize the site of proteolysis, we have established an assay with living human cancer cells in which we image cleavage of quenched-fluorescent basement membrane type IV collagen (DQ-collagen
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some
Functional imaging of proteolysis: stromal and inflammatory cells increase tumor proteolysis.
Journal:Mol Imaging
PubMed ID:14649059
'The underlying basement membrane is degraded during progression of breast and colon carcinoma. Thus, we imaged degradation of a quenched fluorescent derivative of basement membrane type IV collagen (DQ-collagen IV) by living human breast and colon tumor spheroids. Proteolysis of DQ-collagen IV by HCT 116 and HKh-2 human colon tumor