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Citations & References (50)
Invitrogen™
DQ™ Gelatin From Pig Skin, Fluorescein Conjugate - Special Packaging
当社のDQゼラチンは、プロテアーゼ活性をin vitroで高感度に検出するために使用できる蛍光性基質です。この基質は、強く消光されたフルオレセイン標識ゼラチンで構成されており、以前は当社の定評あるEnzChekゼラチナーゼ／コラゲナーゼアッセイキット（E-12055）でのみ入手可能でした。タンパク分解に伴い、輝度の高い緑色の蛍光が現れるため詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| D12054 | 5 x 1 mg |
製品番号（カタログ番号） D12054
価格（JPY）お問い合わせください ›
78,800
Each
数量:
5 x 1 mg
当社のDQゼラチンは、プロテアーゼ活性をin vitroで高感度に検出するために使用できる蛍光性基質です。この基質は、強く消光されたフルオレセイン標識ゼラチンで構成されており、以前は当社の定評あるEnzChekゼラチナーゼ／コラゲナーゼアッセイキット（E-12055）でのみ入手可能でした。タンパク分解に伴い、輝度の高い緑色の蛍光が現れるため、酵素活性の測定に使用できます。蛍光強度の増加は、蛍光マイクロプレートリーダーまたはフルオロメーターでモニタリングできます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
製品ラインDQ
数量5 x 1 mg
出荷条件室温
基質プロテアーゼ基質
検出法蛍光
形状粉末
Substrate Propertiesタンパク質ベースの基質
Target Enzymeメタロプロテイナーゼ
Unit SizeEach
組成および保存条件
フリーザー（-5～-30度）に保存し、遮光してください。
引用および参考文献 (50)
引用および参考文献
Abstract
Gelatin in situ zymography on fixed, paraffin-embedded tissue: zinc and ethanol fixation preserve enzyme activity.
Journal:J Histochem Cytochem
PubMed ID:19755718
In situ zymography is a method for the detection and localization of enzymatic activity in tissue sections. This method is used with frozen sections because routine fixation of tissue in neutral-buffered formalin inhibits enzyme activity. However, frozen sections present with poor tissue morphology, making precise localization of enzymatic activity difficult
Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2.
Journal:J Biol Chem
PubMed ID:12454020
'Primary brain tumors (gliomas) have the unusual ability to diffusely infiltrate the normal brain thereby evading surgical treatment. Chlorotoxin is a scorpion toxin that specifically binds to the surface of glioma cells and impairs their ability to invade. Using a recombinant His-Cltx we isolated and identified the principal Cltx receptor
Authentic matrix vesicles contain active metalloproteases (MMP). a role for matrix vesicle-associated MMP-13 in activation of transforming growth factor-beta.
Journal:J Biol Chem
PubMed ID:11145962
'Matrix vesicles (MV) play a key role in the initiation of cartilage mineralization. Although many components in these microstructures have been identified, the specific function of each component is still poorly understood. In this study, we show that metalloproteases (MMP), MMP-2, -9, and -13 are associated with MV isolated from
Molecular proximity of seprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on beta1 integrins and the cytoskeleton.
Journal:Carcinogenesis
PubMed ID:12376466
'Previous studies have shown that several proteolytic enzymes are associated with membrane protrusions at the leading edge of migrating tumor cells. In this study we demonstrate that seprase and the urokinase plasminogen activator receptor (uPAR), co-localize in the plasma membrane of LOX malignant melanoma cells. Cells were labeled with fluorochrome-conjugated
Pancreatic trypsin increases matrix metalloproteinase-9 accumulation and activation during acute intestinal ischemia-reperfusion in the rat.
Journal:Am J Pathol
PubMed ID:15111317
'Ischemia-reperfusion of the intestine produces a set of inflammatory mediators, the origin of which has recently been shown to involve pancreatic digestive enzymes. Matrix metalloproteinase-9 (MMP-9) participates in a variety of inflammatory processes including myocardial, hepatic, and pancreatic ischemia-reperfusion. In the present study, we explore the role of neutrophil-derived MMP-9