DiIC₁₈(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)

DiIC<sub>18</sub>(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)

Citations & References (11)

Invitrogen™

DiIC₁₈(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)

遠赤色蛍光を示す脂溶性カルボシアニンであるDiIC18(5)-DSには、水溶性を向上させるスルホン酸基が含まれています。水中では弱い蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が非常に高くなります。このDiIアナログに組み込まれたスルホン酸基により、水溶性が向上します。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒詳細を見る

製品番号（カタログ番号）	数量
D12730 または、製品番号D-12730	5 mg

製品番号（カタログ番号） D12730

または、製品番号D-12730

価格（JPY）

56,000

Online offer

Ends: 27-Mar-2026

93,400

割引額 37,400 (40%)

お問い合わせください ›

遠赤色蛍光を示す脂溶性カルボシアニンであるDiIC₁₈(5)-DSには、水溶性を向上させるスルホン酸基が含まれています。水中では弱い蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が非常に高くなります。このDiIアナログに組み込まれたスルホン酸基により、水溶性が向上します。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）。色素は、細胞に加えると原形質膜内で横方向に拡散します。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)蛍光顕微鏡

数量5 mg

出荷条件室温

製品タイプDiIC18(5)-DS

SubCellular Localization原形質膜, 原形質膜&脂質, Lipids, Plasma Membrane

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (11)

引用および参考文献

Abstract

Constructing size distributions of liposomes from single-object fluorescence measurements.

Authors:Lohr C, Kunding AH, Bhatia VK, Stamou D,

Journal:Methods Enzymol

PubMed ID:19913166

We describe in detail a simple technique to construct the size distribution of liposome formulations from single-object fluorescence measurements. Liposomes that are fluorescently labeled in their membrane are first immobilized on a surface at dilute densities and then imaged individually using epi-fluorescence microscopy. The integrated intensities of several thousand single ... More

Lipophilic dye staining of Cryptococcus neoformans extracellular vesicles and capsule.

Authors:Nicola AM, Frases S, Casadevall A,

Journal:Eukaryot Cell

PubMed ID:19465562

Cryptococcus neoformans is an encapsulated yeast that causes systemic mycosis in immunosuppressed individuals. Recent studies have determined that this fungus produces vesicles that are released to the extracellular environment both in vivo and in vitro. These vesicles contain assorted cargo that includes several molecules associated with virulence and implicated in ... More

Distribution of liposomes into brain and rat brain tumor models by convection-enhanced delivery monitored with magnetic resonance imaging.

Authors:Saito R, Bringas JR, McKnight TR, Wendland MF, Mamot C, Drummond DC, Kirpotin DB, Park JW, Berger MS, Bankiewicz KS

Journal:Cancer Res

PubMed ID:15059914

Although liposomes have been used as a vehicle for delivery of therapeutic agents in oncology, their efficacy in targeting brain tumors has been limited due to poor penetration through the blood-brain barrier. Because convection-enhanced delivery (CED) of liposomes may improve the therapeutic index for targeting brain tumors, we conducted a ... More

Modular microcircuit organization of the presubicular head-direction map.

Authors:

Journal:Cell Rep

PubMed ID:35417686

Dual HER2 Targeting with Trastuzumab and Liposomal-Encapsulated Doxorubicin (MM-302) Demonstrates Synergistic Antitumor Activity in Breast and Gastric Cancer.

Authors:

Journal:Cancer Res

PubMed ID:26759238

View all DiIC₁₈(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid) citations

DiIC18(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)

よくあるご質問（FAQ）

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

引用および参考文献 (11)

DiIC₁₈(5)-DS (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine-5,5'-Disulfonic Acid)