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DRAQ7 Dyeは、フローサイトメトリーで非生物細胞を除外するために使用される、明るく使いやすい蛍光DNA色素です。この色素は、599/644 nmで最大励起、678/697 nmで最大蛍光を示します。
•室温で安定
• 洗浄ステップを必要としない死細胞の迅速な染色
• UV励起なし、PEとの発光スペクトルのオーバーラップなし
DRAQ7 Dyeは、死細胞や透過性細胞の二本鎖DNA（dsDNA）を迅速に染色する膜不透過性の色素です。この色素は赤色レーザー線で最適に励起されますが、647～488 nmの波長で励起可能です。dsDNA とのインターカレート時の最大発光波長は697 nmです。
研究用途にのみご使用ください。診断目的には使用できません。
仕様
数量200アッセイ
製品タイプ色素
Unit Size200 assays
組成および保存条件
室温保存。
引用および参考文献 (4)
引用および参考文献
Abstract
Real-time cell viability assays using a new anthracycline derivative DRAQ7®.
Journal:Cytometry A
PubMed ID:23165976
'The exclusion of charged fluorescent dyes by intact cells has become a well-established assay for determining viability of cells. In search for a noninvasive fluorescent probe capable of long-term monitoring of cell death in real-time, we evaluated a new anthracycline derivative DRAQ7. The novel probe does not penetrate the plasma
BET Bromodomain Inhibitor iBET151 Impedes Human ILC2 Activation and Prevents Experimental Allergic Lung Inflammation.
Journal:Front Immunol
PubMed ID:31024538
'Group 2 innate lymphoid cells (ILC2) increase in frequency in eczema and allergic asthma patients, and thus represent a new therapeutic target cell for type-2 immune-mediated disease. The bromodomain and extra-terminal (BET) protein family of epigenetic regulators are known to support the expression of cell cycle and pro-inflammatory genes during
Lysosomotropic challenge of mast cells causes intra-granular reactive oxygen species production.
Journal:Cell Death Discov
PubMed ID:31123601
'Mast cells contribute to the pathology of allergic and other disorders. Strategies to interfere with harmful mast cell-related activities are therefore warranted. Previously we established a principle for inducing selective apoptosis of mast cells, by the use of lysosomotropic agents that cause secretory granule permeabilization, leading to production of reactive
Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells.
Journal:Cell Death Dis
PubMed ID:30952835
The 78-kDa glucose-regulated protein (GRP78) is an ubiquitously expressed endoplasmic reticulum chaperone, with a central role in maintaining protein homeostasis. Recently, an alternative role for GRP78 under stress conditions has been proposed, with stress-induced extracellular secretion and translocation of GRP78 to the cell surface where it acts as a multifunctional