DiO'; DiOC₁₈(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate)

DiO'; DiOC<sub>18</sub>(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate)

Citations & References (188)

Invitrogen™

DiO'; DiOC₁₈(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate)

緑色蛍光を示す脂溶性カルボシアニンであるDiOC18(3)は、脂溶性トレーサーとして広く使用されています。水中では弱い蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が非常に高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）。色素は詳細を見る

製品番号（カタログ番号）	数量
D275	100 mg

製品番号（カタログ番号） D275

価格（JPY）

93,400

お問い合わせください ›

100 mg

緑色蛍光を示す脂溶性カルボシアニンであるDiOC₁₈(3)は、脂溶性トレーサーとして広く使用されています。水中では弱い蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が非常に高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）。色素は、細胞に加えると原形質膜内で横方向に拡散します。

研究用にのみ使用できます。診断用には使用いただけません。

色Green

検出法蛍光

発光501 nm

励起波長域484 nm

使用対象 (装置)蛍光顕微鏡

数量100 mg

出荷条件室温

製品タイプDiO DiOC18(3)

SubCellular Localization細胞膜&脂質, Lipids

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C₁₈ and the other cell line with green fluorescent DiO C₁₈. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all DiO'; DiOC₁₈(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate) FAQs

引用および参考文献 (188)

引用および参考文献

Abstract

Subzonal organization of olfactory sensory neurons projecting to distinct glomeruli within the mouse olfactory bulb.

Authors:Levai O, Breer H, Strotmann J

Journal:J Comp Neurol

PubMed ID:12619077

'Olfactory sensory neurons located in the nasal neuroepithelium send their axons directly into the olfactory bulb, where they contact the dendrites of second-order neurons in specialized spherical structures called glomeruli; each sensory neuron projects to a single glomerulus. All neurons expressing the same odorant receptor gene are confined to distinct ... More

Direct membrane protein-DNA interactions required early in nuclear envelope assembly.

Authors:Ulbert S, Platani M, Boue S, Mattaj IW

Journal:J Cell Biol

PubMed ID:16717124

'Among the earliest events in postmitotic nuclear envelope (NE) assembly are the interactions between chromatin and the membranes that will fuse to form the NE. It has been proposed that interactions between integral NE proteins and chromatin proteins mediate initial membrane recruitment to chromatin. We show that several transmembrane NE ... More

Antigen-induced translocation of PKC-theta to membrane rafts is required for T cell activation.

Authors:Bi K, Tanaka Y, Coudronniere N, Sugie K, Hong S, van Stipdonk MJ, Altman A

Journal:Nat Immunol

PubMed ID:11376344

'Protein kinase C-theta (PKC-theta) is essential for mature T cell activation; however, the mechanism by which it is recruited to the TCR signaling machinery is unknown. Here we show that T cell stimulation by antibodies or peptide-major histocompatibility complex (MHC) induces translocation of PKC-theta to membrane lipid rafts, which localize ... More

Beta-very low density lipoprotein is sequestered in surface-connected tubules in mouse peritoneal macrophages.

Authors:Myers JN, Tabas I, Jones NL, Maxfield FR

Journal:J Cell Biol

PubMed ID:8253839

'beta-very low density lipoprotein (VLDL) is a large lipoprotein with multiple apoprotein E (apoE) molecules that bind to the LDL receptors on mouse macrophages. Even though they bind to the same receptor, the endocytic processing of beta-VLDL differs from low density lipoprotein (LDL). LDL is rapidly delivered to perinuclear lysosomes ... More

Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.

Authors:Brieher WM, Gumbiner BM

Journal:J Cell Biol

PubMed ID:8034750

'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More

View all DiO'; DiOC₁₈(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate) citations

DiO'; DiOC18(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate)

よくあるご質問（FAQ）

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

引用および参考文献 (188)

DiO'; DiOC₁₈(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate)