C₁₂FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)

C<sub>12</sub>FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)

Citations & References (34)

Invitrogen™

C₁₂FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)

β-ガラクトシダーゼ基質、C12FDG は、12-カーボン親油性部分を含むように共有結合的に改変されています。細胞内に入ると、β-ガラクトシダーゼによって基質が開裂され、細胞によって良好に保持される蛍光生成物が生成されます。これはおそらく、細胞膜内に好酸球性テールが組み込まれていることによって実現されます詳細を見る

製品番号（カタログ番号）	数量
D2893	5 mg

製品番号（カタログ番号） D2893

価格（JPY）

79,100

Ends: 27-Mar-2026

131,900

割引額 52,800 (40%)

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β-ガラクトシダーゼ基質、C₁₂FDG は、12-カーボン親油性部分を含むように共有結合的に改変されています。細胞内に入ると、β-ガラクトシダーゼによって基質が開裂され、細胞によって良好に保持される蛍光生成物が生成されます。これはおそらく、細胞膜内に好酸球性テールが組み込まれていることによって実現されます。C₁₂FDGは、ImaGene™ Greenキットの主要コンポーネントです。

研究用にのみ使用できます。診断用には使用いただけません。

励起／発光490⁄514

数量5 mg

出荷条件室温

基質β-Gal基質

検出法蛍光

Substrate Properties化学基質

Unit SizeEach

組成および保存条件

冷凍庫（-5～-30℃）に保存。

引用および参考文献 (34)

引用および参考文献

Abstract

Rapid flow cytometric method for measuring senescence associated beta-galactosidase activity in human fibroblasts.

Authors:Noppe G, Dekker P, de Koning-Treurniet C, Blom J, van Heemst D, Dirks RW, Tanke HJ, Westendorp RG, Maier AB,

Journal:Cytometry A

PubMed ID:19777541

'Senescence associated-beta-galactosidase (SA-beta-gal) activity is a widely used marker for cellular senenescence. SA-beta-gal activity is routinely detected cytochemically, manually discriminating negative from positive cells. This method is time-consuming, subjective and therefore prone to operator-error. We aimed to optimize a flow cytometric method described by other workers using endothelial cells to ... More

Optical imaging fiber-based single live cell arrays: a high-density cell assay platform.

Authors:Biran I, Walt DR

Journal:Anal Chem

PubMed ID:12141663

'A high-density, ordered array containing thousands of microwells is fabricated on an optical imaging fiber. Each individually addressable microwell is used to accommodate a single living cell. A charged coupled device (CCD) detector is employed to monitor and spatially resolve the fluorescence signals obtained from each individual cell, allowing simultaneous ... More

An optimized electroporation protocol applicable to a wide range of cell lines.

Authors:Baum C, Forster P, Hegewisch-Becker S, Harbers K

Journal:Biotechniques

PubMed ID:7873174

'A number of transfection methods for mammalian cells are available; however, many cell lines may appear resistant to efficient transfection, or at best, necessitate lengthy optimization procedures in recommended protocols. We describe here an electroporation protocol that yields highly efficient gene transfer (20%-100% of surviving cells) in all 19 cell ... More

Mapping mechanisms and charting the time course of premature cell senescence and apoptosis: lysosomal dysfunction and ganglioside accumulation in endothelial cells.

Authors:Patschan S, Chen J, Gealekman O, Krupincza K, Wang M, Shu L, Shayman JA, Goligorsky MS,

Journal:Am J Physiol Renal Physiol

PubMed ID:17928415

'Endothelial cells subjected to glycated collagen I develop premature senescence within 3-5 days, as revealed by increased senescence-associated beta-galactosidase activity, decreased proliferation, and an increase in cell size. Here, we analyzed the time course and possible mechanisms of this process. Lysosomal integrity studies revealed a rapid collapse of pH gradient ... More

Use of fluorescence-activated cell sorting for rapid isolation of insect cells harboring recombinant baculovirus.

Authors:Sommerfelt MA, Sorscher EJ

Journal:Methods Cell Biol

PubMed ID:7877509

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C12FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)

引用および参考文献 (34)

C₁₂FDG (5-Dodecanoylaminofluorescein Di-β-D-Galactopyranoside)