DiD' oil; DiIC₁₈(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)

DiD' oil; DiIC<sub>18</sub>(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)

Citations & References (69)

Invitrogen™

DiD' oil; DiIC₁₈(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)

遠赤色蛍光を示す脂溶性カルボシアニンであるDiDは、波長の長いDiIアナログです。これは室温では油状であり、水中では弱く蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）詳細を見る

製品番号（カタログ番号）	数量
D307	25 mg

製品番号（カタログ番号） D307

価格（JPY）

38,200

Exklusiv online

Ends: 27-Mar-2026

63,800

割引額 25,600 (40%)

お問い合わせください ›

25 mg

遠赤色蛍光を示す脂溶性カルボシアニンであるDiDは、波長の長いDiIアナログです。これは室温では油状であり、水中では弱く蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）。色素は、細胞に加えると原形質膜内で横方向に拡散します。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

発光665 nm

励起波長域644 nm

使用対象 (装置)蛍光顕微鏡

分子量959.92

数量25 mg

出荷条件室温

製品タイプDiDオイルDiIC18(5) Oil

SubCellular Localization細胞膜&脂質, Lipids

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all DiD' oil; DiIC₁₈(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate) FAQs

引用および参考文献 (69)

引用および参考文献

Abstract

Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.

Authors:Kamoun WS, Chae SS, Lacorre DA, Tyrrell JA, Mitre M, Gillissen MA, Fukumura D, Jain RK, Munn LL,

Journal:Nat Methods

PubMed ID:20581828

Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We ... More

Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.

Authors:Servant G, Weiner OD, Neptune ER, Sedat JW, Bourne HR

Journal:Mol Biol Cell

PubMed ID:10198064

'Persistent directional movement of neutrophils in shallow chemotactic gradients raises the possibility that cells can increase their sensitivity to the chemotactic signal at the front, relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a polarized neutrophil could impose asymmetric sensitivity by increasing the relative strength ... More

Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.

Authors:Brieher WM, Gumbiner BM

Journal:J Cell Biol

PubMed ID:8034750

'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More

Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.

Authors:Evans CL, Potma EO, Puoris'haag M, Côté D, Lin CP, Xie XS

Journal:Proc Natl Acad Sci U S A

PubMed ID:16263923

'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with ... More

In vivo migration of dendritic cells differentiated in vitro: a chimpanzee model.

Authors:Barratt-Boyes SM, Watkins SC, Finn OJ

Journal:J Immunol

PubMed ID:9144465

'Dendritic cells with potent Ag-presenting function can be propagated from peripheral blood using recombinant cytokines, and these cells have potential usefulness as immunotherapeutic agents in the treatment of cancer and other disease states. However, it is not known if these in vitro differentiated dendritic cells have the capacity to migrate ... More

View all DiD' oil; DiIC₁₈(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate) citations

DiD' oil; DiIC18(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)

よくあるご質問（FAQ）

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

引用および参考文献 (69)

DiD' oil; DiIC₁₈(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)