DiIC₁₂(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)

DiIC<sub>12</sub>(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)

Citations & References (53)

Invitrogen™

DiIC₁₂(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)

赤橙色蛍光を示す脂溶性カルボシアニンであるDiIC12(3)は、DiIよりも鎖が短く、細胞膜に取り込みやすいDiI（DilC18(3)）アナログです。水中では弱い蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が非常に高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります詳細を見る

製品番号（カタログ番号）	数量
D383	100 mg

製品番号（カタログ番号） D383

価格（JPY）

93,400

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100 mg

赤橙色蛍光を示す脂溶性カルボシアニンであるDiIC₁₂(3)は、DiIよりも鎖が短く、細胞膜に取り込みやすいDiI（DilC₁₈(3)）アナログです。水中では弱い蛍光を発しますが、細胞膜に取り込まれると強い蛍光を発し、光安定性が非常に高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）。色素は、細胞に加えると原形質膜内で横方向に拡散します。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)蛍光顕微鏡

数量100 mg

出荷条件室温

製品タイプDiIC12(3)

SubCellular Localization細胞膜&脂質, Lipids

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

View all DiIC₁₂(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate) FAQs

引用および参考文献 (53)

引用および参考文献

Abstract

Authors:

Journal:

PubMed ID:10404414

Reticulated lipid probe fluorescence reveals MDCK cell apical membrane topography.

Authors:Colarusso P, Spring KR

Journal:Biophys J

PubMed ID:11806917

High spatial resolution confocal microscopy of young MDCK cells stained with the lipophilic probe 1,1'-dihexadecyl-3,3,3',3'- tetramethylindocarbocyanine perchlorate (DiIC(16)) revealed a reticulated fluorescence pattern on the apical membrane. DiIC(16) was delivered as crystals to live cells to minimize possible solvent perturbations of the membrane lipids. The ratio of the integrated fluorescence ... More

Fluorescence imaging of the desorption of dye from fused silica versus silica gel.

Authors:Ludes MD, Anthony SR, Wirth MJ

Journal:Anal Chem

PubMed ID:12964753

The desorption rate constants for a cationic dye from strong adsorption sites are compared for the same chromatographic interface but for two different substrates, fused silica and chromatographic silica gel. The dye is 1,1'-didodecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate (DiI). The interface consists of acetonitrile and a hydrocarbon monolayer (C8) covalently bound to the ... More

Distance measurements within a concatamer of the plasma membrane Cl?/HCO3? exchanger, AE1.

Authors:Basu A, Mazor S, Casey JR,

Journal:Biochemistry

PubMed ID:20828148

'AE1, which exists in the erythrocyte plasma membrane as a noncovalent dimer, facilitates transmembrane Cl?/HCO3? exchange. Here a concatamer of AE1 (two AE1 monomers fused via a two-residue linker to form an intramolecular dimer) was designed to facilitate fluorescence resonance energy transfer (FRET) studies. The concatameric protein (AE1·AE1) was expressed ... More

Characterization of myenteric interneurons with somatostatin immunoreactivity in the guinea-pig small intestine.

Authors:Song ZM, Brookes SJ, Ramsay GA, Costa M

Journal:Neuroscience

PubMed ID:9276502

'The projections, connections, morphology and electrophysiological features of the myenteric interneurons with somatostatin immunoreactivity in the guinea-pig small intestine have been established using retrograde tracing, immunohistochemistry, confocal microscopy and intracellular recording. After application of the fluorescent dye, 1,1''-didodecyl-3,3,3'',3''-tetramethyl indocarbocyanine perchlorate (DiI), to the myenteric plexus, up to 900 nerve cell ... More

View all DiIC₁₂(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate) citations

DiIC12(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)

よくあるご質問（FAQ）

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?

I want to label two cell populations and then perform a cell fusion assay. Which reagents are best for imaging this?

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

引用および参考文献 (53)

DiIC₁₂(3) (1,1'-Didodecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate)