Search
Citations & References (9)
Invitrogen™
DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)
ガラクトシダーゼ基質であるDDAOガラクトシドは、633 nmレーザーで励起できる加水分解生成物を生成します（励起／発光の最大値は約645/660）。基質自体は蛍光性ですが（励起／発光の最大値は約460/610 nm）、基質と加水分解生成物の差は200詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| D6488 または、製品番号D-6488 | 5 mg |
製品番号（カタログ番号） D6488
または、製品番号D-6488
価格（JPY）お問い合わせください ›
57,600
Online offer
Ends: 27-Mar-2026
96,000割引額 38,400 (40%)
Each
数量:
5 mg
ガラクトシダーゼ基質であるDDAOガラクトシドは、633 nmレーザーで励起できる加水分解生成物を生成します（励起／発光の最大値は約645/660）。基質自体は蛍光性ですが（励起／発光の最大値は約460/610 nm）、基質と加水分解生成物の差は200 nmを超えているため、2つの種を容易に識別できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
励起／発光645⁄660
分子量470.31
数量5 mg
出荷条件室温
基質β-Gal基質
検出法蛍光
Substrate Properties化学基質
Unit SizeEach
組成および保存条件
フリーザー（-5～-30度）に保存し、遮光してください。
引用および参考文献 (9)
引用および参考文献
Abstract
Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia.
Journal:Mol Imaging
PubMed ID:17711777
'Reporter gene techniques have been applied toward studying the physiologic phenomena associated with tumor hypoxia, a negative prognostic indicator. The purpose of this study was to assess the potential adverse effects of hypoxic conditions on the effectiveness of four commonly used reporter genes: Renilla luciferase, monomeric red fluorescent protein, thymidine
In vivo imaging of beta-galactosidase activity using far red fluorescent switch.
Journal:Cancer Res
PubMed ID:14996712
'beta-Galactosidase (beta-gal) has been widely used as a transgene reporter enzyme, and several substrates are available for its in vitro detection. The ability to image beta-gal expression in living animals would further extend the use of this reporter. Here we show that DDAOG, a conjugate of beta-galactoside and 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) (DDAO),
beta-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOG.
Journal:Anal Biochem
PubMed ID:19103143
beta-Galactosidase (beta-gal) is commonly used as a reporter gene in biological research, and a wide variety of substrates have been developed to assay its activity. One substrate, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-d-galactopyranoside (DDAOG), can be cleaved by beta-gal to produce 7-hydroxy-9H(I,3-dichloro-9,9-dimethylacridin-2-one) (DDAO). On excitation, DDAO generates a far-red-shifted fluorescent signal. Using this substrate,
Enzymatic detection of protein translocation.
Journal:Nat Methods
PubMed ID:15973423
Fundamental to eukaryotic cell signaling is the regulation of protein function by directed localization. Detection of these events has been largely qualitative owing to the limitations of existing technologies. Here we describe a method for quantitatively assessing protein translocation using proximity-induced enzyme complementation. The complementation assay for protein translocation (CAPT)
Quantification of beta-galactosidase activity after non-viral transfection in vivo.
Journal:J Control Release
PubMed ID:12932652
The limited efficacy of non-viral gene delivery systems currently hampers their wider therapeutic use. In order to further develop novel gene delivery systems, it is important to quantify their efficacy. Many reporter gene assays have limitations when being used to quantify expression in vivo. We have developed a simple assay