DiD' solid; DiIC₁₈(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)

DiD' solid; DiIC<sub>18</sub>(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)

Citations & References (71)

Invitrogen™

DiD' solid; DiIC₁₈(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)

遠赤色蛍光を示す脂溶性カルボシアニンであるDiDは、波長の長いDiIアナログです。膜に対して結晶を直接容易に適用できる固体形態で供給され、水中では弱く蛍光を発しますが、膜に組み込むと強い蛍光を発し、光安定性が非常に高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）詳細を見る

製品番号（カタログ番号）	数量
D7757	10 mg

製品番号（カタログ番号） D7757

価格（JPY）

51,600

お問い合わせください ›

10 mg

遠赤色蛍光を示す脂溶性カルボシアニンであるDiDは、波長の長いDiIアナログです。膜に対して結晶を直接容易に適用できる固体形態で供給され、水中では弱く蛍光を発しますが、膜に組み込むと強い蛍光を発し、光安定性が非常に高くなります。脂質環境では吸光係数が非常に高くなり、短い励起状態の寿命が短くなります（∼1ナノ秒）。色素は、細胞に加えると原形質膜内で横方向に拡散します。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)蛍光顕微鏡

数量10 mg

出荷条件室温

製品タイプDiD Solid DiIC18（5）固体

SubCellular Localization細胞膜&脂質, Lipids

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C₁₈ and the other cell line with green fluorescent DiO C₁₈. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

If the tracer you chose is a lipophilic dye and fix with methanol, the lipids are lost with the methanol. If you have to use methanol fixation then choose a tracer that will covalently bind to proteins in the neurons.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all DiD' solid; DiIC₁₈(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt) FAQs

引用および参考文献 (71)

引用および参考文献

Abstract

Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.

Authors:Kamoun WS, Chae SS, Lacorre DA, Tyrrell JA, Mitre M, Gillissen MA, Fukumura D, Jain RK, Munn LL,

Journal:Nat Methods

PubMed ID:20581828

Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We ... More

Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.

Authors:Servant G, Weiner OD, Neptune ER, Sedat JW, Bourne HR

Journal:Mol Biol Cell

PubMed ID:10198064

'Persistent directional movement of neutrophils in shallow chemotactic gradients raises the possibility that cells can increase their sensitivity to the chemotactic signal at the front, relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a polarized neutrophil could impose asymmetric sensitivity by increasing the relative strength ... More

Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.

Authors:Brieher WM, Gumbiner BM

Journal:J Cell Biol

PubMed ID:8034750

'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More

Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.

Authors:Evans CL, Potma EO, Puoris'haag M, Côté D, Lin CP, Xie XS

Journal:Proc Natl Acad Sci U S A

PubMed ID:16263923

'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with ... More

In vivo migration of dendritic cells differentiated in vitro: a chimpanzee model.

Authors:Barratt-Boyes SM, Watkins SC, Finn OJ

Journal:J Immunol

PubMed ID:9144465

'Dendritic cells with potent Ag-presenting function can be propagated from peripheral blood using recombinant cytokines, and these cells have potential usefulness as immunotherapeutic agents in the treatment of cancer and other disease states. However, it is not known if these in vitro differentiated dendritic cells have the capacity to migrate ... More

View all DiD' solid; DiIC₁₈(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt) citations

DiD' solid; DiIC18(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)

よくあるご質問（FAQ）

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

引用および参考文献 (71)

DiD' solid; DiIC₁₈(5) solid (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt)