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Citations & References (225)
Invitrogen™
Ethidium Homodimer-1 (EthD-1)
細胞不透過性の視覚的インジケーターであるエチジウムのホモ二量体-1（EthD-1）は、DNAに結合するまで弱い蛍光を発し、赤色の蛍光を発する高親和性核酸染色剤です（最大励起／最大発光約528/617）。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| E1169 | 1 mg |
製品番号（カタログ番号） E1169
価格（JPY）お問い合わせください ›
103,700
Each
数量:
1 mg
細胞不透過性の視覚的インジケーターであるエチジウムのホモ二量体-1（EthD-1）は、DNAに結合するまで弱い蛍光を発し、赤色の蛍光を発する高親和性核酸染色剤です（最大励起／最大発光約528/617）。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
色オレンジ
概要Ethidium Homodimer-1 (EthD-1)
検出法蛍光
染色剤タイプ細胞非透過性
発光617 nm
励起波長域528⁄617
使用対象（アプリケーション）Cell staining assays
使用対象 (装置)蛍光顕微鏡
数量1 mg
出荷条件室温
標識タイプFluorescent Dye
製品タイプエチジウムホモダイマー-1
SubCellular Localization核、核酸, Nucleus
Unit SizeEach
組成および保存条件
フリーザー(-5°C∼-30°C)に保存し、遮光してください。
よくあるご質問（FAQ）
I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?
引用および参考文献 (225)
引用および参考文献
Abstract
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:9110282
OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:8930888
OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain
A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels.
Journal:Proc Natl Acad Sci U S A
PubMed ID:2339125
The complex between double-stranded DNA and ethidium homodimer (5,5'-diazadecamethylene)bis(3,8-diamino-6-phenylphenanthridini um) cation, formed at a ratio of 1 homodimer per 4 or 5 base pairs, is stable in agarose gels under the usual conditions for electrophoresis. This unusual stability allows formation of the complex before electrophoresis and then separation and detection
Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.
Journal:Exp Neurol
PubMed ID:14769360
Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept
Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
Journal:J Biol Chem
PubMed ID:16785236
'Although indirubin is known to exhibit anti-cancer and anti-inflammatory activities, very little is known about its mechanism of action. In this study, we investigated whether indirubin mediates its effects through interference with the NF-kappaB pathway. As examined by the DNA binding of NF-kappaB, we found that indirubin suppressed tumor necrosis