EnzChek™ Lysozyme Assay Kit

Citations & References (11)

Invitrogen™

EnzChek™ Lysozyme Assay Kit

製品番号（カタログ番号）	数量
E22013	400 Assays

製品番号（カタログ番号） E22013

価格（JPY）

48,100

キャンペーン価格

Ends: 27-Mar-2026

80,200

割引額 32,100 (40%)

お問い合わせください ›

400 Assays

EnzChek™リゾチームアッセイキットは、溶液中のリゾチーム活性を測定するためのシンプルで高感度なアッセイを提供します。リゾチーム活性による蛍光強度の増加は、フルオロメーターまたは蛍光マイクロプレートリーダーを使用して測定します。

蛍光マイクロプレートアッセイの全製品ラインアップをご覧ください。

•20 U/mLという低いリゾチーム活性濃度を検出します
• 標準的なフルオレセイン（FITC）励起／発光設定を使用します
• リゾチーム活性を連続的に測定できるフォーマットです

このアッセイは、革新的なアプローチを利用してリゾチーム活性を測定します。ミクロコッカスリゾデイクティカスの細胞壁が使用されており、蛍光シグナルの消光を引き起こすフルオレセインで十分に標識されています。活性リゾチーム酵素は、ムコ多糖類細胞壁のN-アセチルムラミン酸とN-アセチル-D-グルコサミン残基間のb（1-4）-グリコシド結合を加水分解し、消光を緩和し、リゾチーム活性に比例した蛍光強度を劇的に増加させます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光強度

数量400 Assays

出荷条件室温

基質特性脂質ベースの基質

基質タイプリゾチーム基質

標的酵素Lysozyme

使用対象（アプリケーション）リゾチームアッセイ

使用対象 (装置)蛍光マイクロプレートリーダー

製品ラインEnzChek

製品タイプリゾチームアッセイ

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

How can I limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013)?

To limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013), try lowering the voltage (gain) on the PMT (photomultiplier tube). If you are still seeing signal saturation after lowering the voltage, try a shorter reaction time, and/or lower substrate concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?

The primary amines on the cell wall of Micrococcus lysodeikticus were conjugated to fluorescein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May other buffers be used for the EnzChek Lysozyme assay?

Yes. You may use other buffers that are compatible with the enzyme of interest. The buffer provided is optimal for use with chicken egg white lysozyme. If the buffer has a pH <6, adjust the pH to 7.5 to 8 prior to detection for optimal signal from the fluorescein. The emission of fluorescein drops off at pH <6.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (11)

引用および参考文献

Abstract

Vogesella mureinivorans sp. nov., a peptidoglycan-degrading bacterium from lake water.

Authors:Jørgensen NO, Brandt KK, Nybroe O, Hansen M,

Journal:Int J Syst Evol Microbiol

PubMed ID:19946047

'A novel, non-pigmented, rod-shaped, Gram-negative strain was isolated from mesotrophic lake water in Zealand, Denmark. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium, designated strain 389(T), indicated that the strain belonged to the genus Vogesella and formed a monophyletic group with Vogesella perlucida DS-28(T) (99.1?% nucleotide similarity); ... More

Structure-function analysis of HsiF, a gp25-like component of the type VI secretion system, in Pseudomonas aeruginosa.

Authors:Lossi NS, Dajani R, Freemont P, Filloux A,

Journal:Microbiology

PubMed ID:21873404

'Bacterial pathogens use a range of protein secretion systems to colonize their host. One recent addition to this arsenal is the type VI secretion system (T6SS), which is found in many Gram-negative bacteria. The T6SS involves 12-15 components, including a ClpV-like AAA(+) ATPase. Moreover, the VgrG and Hcp components have ... More

Mechanisms of systemic vasodilation by lysozyme-c in septic shock.

Authors:Gotes J, Kasian K, Jacobs H, Cheng ZQ, Mink SN,

Journal:J Appl Physiol (1985)

PubMed ID:22096116

In septic shock (SS), cardiovascular collapse is caused by the release of inflammatory mediators. We previously found that lysozyme-c (Lzm-S), released from leukocytes, contributed to systemic vasodilation in a canine model of SS. We then delineated the pathway by which this occurs in a canine carotid artery organ bath preparation ... More

Mutations of the Listeria monocytogenes peptidoglycan N-deacetylase and O-acetylase result in enhanced lysozyme sensitivity, bacteriolysis, and hyperinduction of innate immune pathways.

Authors:Rae CS, Geissler A, Adamson PC, Portnoy DA,

Journal:Infect Immun

PubMed ID:21768286

Listeria monocytogenes is a Gram-positive intracellular pathogen that is naturally resistant to lysozyme. Recently, it was shown that peptidoglycan modification by N-deacetylation or O-acetylation confers resistance to lysozyme in various Gram-positive bacteria, including L. monocytogenes. L. monocytogenes peptidoglycan is deacetylated by the action of N-acetylglucosamine deacetylase (Pgd) and acetylated by ... More

Alcohol use disorders affect antimicrobial proteins and anti-pneumococcal activity in epithelial lining fluid obtained via bronchoalveolar lavage.

Authors:Burnham EL, Gaydos J, Hess E, House R, Cooper J,

Journal:Alcohol Alcohol

PubMed ID:20729531

Our overall objective was to examine whether characteristics of epithelial lining fluid (ELF) from subjects with alcohol use disorders (AUDs) obtained via bronchoalveolar lavage (BAL) contribute to their predisposition to pneumococcal pneumonia. We sought to compare the anti-pneumococcal activity of acellular human BAL from subjects with AUDs to matched controls. ... More

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