EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Citations & References (8)

Invitrogen™

EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

ミエロペルオキシダーゼ（MPO）は、その過酸化活性に加えて、過酸化水素（H2O2）と塩化物（Cl-）の次亜塩素酸（HOCl詳細を見る

製品番号（カタログ番号）	数量
E33856	1 Kit

製品番号（カタログ番号） E33856

価格（JPY）

163,700

お問い合わせください ›

1 Kit

ミエロペルオキシダーゼ（MPO）は、その過酸化活性に加えて、過酸化水素（H₂O₂）と塩化物（Cl^-）の次亜塩素酸（HOCl）への変換を触媒するユニークなペルオキシダーゼです。EnzChek™ミエロペルオキシダーゼ（MPO）活性アッセイキットは、溶液中および細胞溶解物中のMPOの塩素化活性と過酸化活性の両方を測定するためのアッセイを提供します。塩素化の検出のため、キットには非蛍光性3'-（p-アミノフェニル）フルオレセイン（APF）が含まれており、次亜塩素酸（^-OCl）によって選択的に開裂され、フルオレセインが得られます。過酸化は、非蛍光性Amplex™UltraRed試薬（A36006）を用いて検出されますが、これは、H₂O₂によって生成された酸化還元中間体MPO-IとMPO-IIによって酸化され、蛍光生成物を形成しますEnzChek™ミエロペルオキシダーゼ活性アッセイキットは、広いダイナミックレンジ（1.5～200 ng/mL）にわたり室温でこれらの活性の連続検出用に使用することができます。このキットは、速度（30分）、感度、mix-and-read（混和して測定）の利便性により、MPO活性の測定およびMPO特異的阻害剤のハイスループットスクリーニングに最適です。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光強度

数量1 Kit

出荷条件湿氷

基質特性化学基質

標的酵素ミエロペルオキシダーゼ

使用対象（アプリケーション）ミエロペルオキシダーゼ（MPO）活性アッセイ

製品ラインEnzChek

製品タイプミエロペルオキシダーゼ（MPO）アッセイ

Unit SizeEach

組成および保存条件

冷蔵庫（2℃～8℃）に保存し、遮光してください。

よくあるご質問（FAQ）

What is the dynamic range of the EnzChek Myeloperoxidase (MPO) Activity Assay Kit (Cat. No. E33856)?

The EnzChek Myeloperoixdase Activity Assay Kit (Cat. No. E33856) can detect myeloperoxidase activity at a broad dynamic range of 1.5 to 200 ng/mL.

What can interfere with the EnzChek Myeloperoxidase (MPO) Activity assay?

MPO is inhibited by azide, diclofenac, methimazole, quercetin, rutin, and salicylhydroxamic acid. Make certain that samples do not include sodium azide. Endogenous catalases can interfere with the assay; catalase activity may be inhibited by using 3-amino-1,2,4-triazole. Detergents, common components in cell lysis buffers, should be avoided; use freeze/thawing and/or mechanical methods to lyse cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (8)

引用および参考文献

Abstract

Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice.

Authors:Deng Y, Edin ML, Theken KN, Schuck RN, Flake GP, Kannon MA, DeGraff LM, Lih FB, Foley J, Bradbury JA, Graves JP, Tomer KB, Falck JR, Zeldin DC, Lee CR,

Journal:FASEB J

PubMed ID:21059750

Cytochrome P-450 (CYP)-derived epoxyeicosatrienoic acids (EETs) possess potent anti-inflammatory effects in vitro. However, the effect of increased CYP-mediated EET biosynthesis and decreased soluble epoxide hydrolase (sEH, Ephx2)-mediated EET hydrolysis on vascular inflammation in vivo has not been rigorously investigated. Consequently, we characterized acute vascular inflammatory responses to endotoxin in transgenic ... More

G-CSF treatment of severe congenital neutropenia reverses neutropenia but does not correct the underlying functional deficiency of the neutrophil in defending against microorganisms.

Authors:Donini M, Fontana S, Savoldi G, Vermi W, Tassone L, Gentili F, Zenaro E, Ferrari D, Notarangelo LD, Porta F, Facchetti F, Notarangelo LD, Dusi S, Badolato R

Journal:Blood

PubMed ID:17311988

'The treatment of children affected by severe congenital neutropenia (SCN) with G-CSF strongly reduces the risk of sepsis by reversing neutropenia. However, SCN patients who respond to the treatment with the growth factor still have an elevated risk of succumbing to sepsis. Because the disease is usually caused by heterozygous ... More

Targeted deletion of tumor suppressor PTEN augments neutrophil function and enhances host defense in neutropenia-associated pneumonia.

Authors:Li Y, Jia Y, Pichavant M, Loison F, Sarraj B, Kasorn A, You J, Robson BE, Umetsu DT, Mizgerd JP, Ye K, Luo HR,

Journal:Blood

PubMed ID:19286998

'Neutropenia and related infections are the most important dose-limiting toxicities in anticancer chemotherapy and radiotherapy. In this study, we explored a new strategy for augmenting host defense in neutropenia-related pneumonia. Phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) signaling in neutrophils was elevated by depleting PTEN, a phosphatidylinositol 3''-phosphatase that hydrolyzes PtdIns(3,4,5)P(3). In myeloid-specific PTEN knockout ... More

Inflammation-induced, 3'UTR-dependent translational inhibition of Hsp70 mRNA impairs intestinal homeostasis.

Authors:Hu S, Zhu X, Triggs JR, Tao Y, Wang Y, Lichtenstein L, Bissonnette M, Musch MW, Chang EB,

Journal:Am J Physiol Gastrointest Liver Physiol

PubMed ID:19299581

'Although the inducible heat shock protein 70 (Hsp70) is essential for maintaining intestinal homeostasis in colitis, it is translationally downregulated in inflamed colonic mucosa, paradoxically rendering the gut more susceptible to injury. We examined the basis for this process by analyzing the role of untranslated regions (UTR) of Hsp70 mRNA ... More

Functional consequence of positive selection revealed through rational mutagenesis of human myeloperoxidase.

Authors:Loughran NB, Hinde S, McCormick-Hill S, Leidal KG, Bloomberg S, Loughran ST, O'Connor B, O'Fágáin C, Nauseef WM, O'Connell MJ,

Journal:Mol Biol Evol

PubMed ID:22355012

'Myeloperoxidase (MPO) is a member of the mammalian heme peroxidase (MHP) multigene family. Whereas all MHPs oxidize specific halides to generate the corresponding hypohalous acid, MPO is unique in its capacity to oxidize chloride at physiologic pH to produce hypochlorous acid (HOCl), a potent microbicide that contributes to neutrophil-mediated host ... More

View all EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit citations