Authors:Lu S, Wang X, Lu Q, Hu X, Uppal N, Omenetto FG, Kaplan DL,
Journal:Biomacromolecules
PubMed ID:19323497
'Material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. We demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. Enzymes (glucose ... More
Determination of lipoprotein lipase activity using a novel fluorescent lipase assay.
Authors:Basu D, Manjur J, Jin W,
Journal:J Lipid Res
PubMed ID:21270098
A novel, real-time, homogeneous fluorogenic lipoprotein lipase (LPL) assay was developed using a commercially available substrate, the EnzChek lipase substrate, which is solubilized in Zwittergent. The triglyceride analog substrate does not fluoresce, owing to apposition of fluorescent and fluorescent quenching groups at the sn-1 and sn-2 positions, respectively, fluorescence becoming ... More