ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

Citations & References (28)

Invitrogen™

ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

ER-Tracker™ Green色素は細胞透過性の生細胞染色で、提供されたプロトコルを使用して染色すると、ホルムアルデヒドで固定した後に染色パターンが部分的に保持されるため、小胞体（ER）に対して高い選択性を示します。この染色剤は、緑色蛍光を示すBODIPY™ FL色素とグリベンクラミドで構成されています詳細を見る

製品番号（カタログ番号）	数量
E34251	100 μg

製品番号（カタログ番号） E34251

価格（JPY）

90,000

お問い合わせください ›

100 μg

ER-Tracker™ Green色素は細胞透過性の生細胞染色で、提供されたプロトコルを使用して染色すると、ホルムアルデヒドで固定した後に染色パターンが部分的に保持されるため、小胞体（ER）に対して高い選択性を示します。この染色剤は、緑色蛍光を示すBODIPY™ FL色素とグリベンクラミドで構成されています。グリアンクラミド（グリブリド）は、ER上で顕著になるATP感受性K⁺チャネルのサルフォルユアリア受容体に結合します。グリベンクラミドの薬理活性はER機能に影響を及ぼす可能性があります。特定の特殊な細胞タイプでサルフォルユアリア受容体の発現が変動すると、非ER標識が生じる場合があります。

研究用にのみ使用できます。診断用には使用いただけません。

色Green

検出法蛍光

使用対象 (装置)蛍光顕微鏡

製品ラインBODIPY, ER-Tracker

数量100 μg

出荷条件室温

標識タイプBODIPY Dyes

製品タイプ色素

SubCellular Localization小胞体

Unit SizeEach

組成および保存条件

フリーザー(-5°C∼-30°C)に保存し、遮光してください。

よくあるご質問（FAQ）

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (28)

引用および参考文献

Abstract

Morphological localisation of sulfonylurea receptor 1 in endocrine cells of human, mouse and rat pancreas.

Authors:Guiot Y, Stevens M, Marhfour I, Stiernet P, Mikhailov M, Ashcroft SJ, Rahier J, Henquin JC, Sempoux C,

Journal:Diabetologia

PubMed ID:17593344

'AIMS/HYPOTHESIS: Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive K channels in beta cells. Morphological methods (immunohistochemistry and sulfonylurea binding) were used to establish the cellular and subcellular location of SUR1 in human and rodent islets. RESULTS: In the human, mouse and rat pancreas, all endocrine cells of ... More

Live intracellular super-resolution imaging using site-specific stains.

Authors:Carlini L, Manley S,

Journal:

PubMed ID:24079385

'Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several ... More

Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis.

Authors:Mirnikjoo B, Balasubramanian K, Schroit AJ,

Journal:J Biol Chem

PubMed ID:19561081

One of the hallmarks of apoptosis is the redistribution of phosphatidylserine (PS) from the inner-to-outer plasma membrane (PM) leaflet, where it functions as a ligand for phagocyte recognition and the suppression of inflammatory responses. The mechanism by which apoptotic cells externalize PS has been assumed to involve ... More

Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.

Authors:Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,

Journal:J Biol Chem

PubMed ID:18281291

Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as a means of providing macromolecules for energy generation under conditions of nutrient starvation. In this study we demonstrate that mitochondrial autophagy is induced by hypoxia, that this process requires the hypoxia-dependent factor-1-dependent ... More

Translocation of a phycoerythrin alpha subunit across five biological membranes.

Authors:Gould SB, Fan E, Hempel F, Maier UG, Klösgen RB,

Journal:J Biol Chem

PubMed ID:17702756

Cryptophytes, unicellular algae, evolved by secondary endosymbiosis and contain plastids surrounded by four membranes. In contrast to cyanobacteria and red algae, their phycobiliproteins do not assemble into phycobilisomes and are located within the thylakoid lumen instead of the stroma. We identified two gene families encoding phycoerythrin alpha and light-harvesting complex ... More

View all ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging citations