概要Ethidium Homodimer-2 (EthD-2) - 1 mM Solution in DMSO
検出法蛍光
染色剤タイプ細胞非透過性
発光624 nm
励起波長域535⁄624
使用対象(アプリケーション)Cell staining assays
使用対象 (装置)蛍光顕微鏡
数量200 μL
出荷条件室温
標識タイプFluorescent Dye
製品タイプエチジウムホモダイマー-2
SubCellular Localization核、核酸, Nucleus
Unit SizeEach
組成および保存条件
フリーザー(-5°C∼-30°C)に保存し、遮光してください。
引用および参考文献 (52)
引用および参考文献
Abstract
Differential effects of deuterium oxide on the fluorescence lifetimes and intensities of dyes with different modes of binding to DNA.
Authors:Sailer BL, Nastasi AJ, Valdez JG, Steinkamp JA, Crissman HA
Journal:J Histochem Cytochem
PubMed ID:9016307
'Deuterium oxide (D2O) increases both the fluorescence lifetime and the fluorescence intensity of the intercalating dyes propidium iodide (PI) and ethidium bromide (EB) when bound to nucleic acid structures. We have used spectroscopic analysis coupled with conventional and phase-sensitive flow cytometry to compare the alterations in intensity and lifetime of ... More
Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.
Authors:Petersen MA, Dailey ME
Journal:Glia
PubMed ID:15042586
'We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead ... More
Calmodulin antagonists differentially affect capacitation-associated protein tyrosine phosphorylation of mouse sperm components.
Authors:Zeng HT, Tulsiani DR
Journal:J Cell Sci
PubMed ID:12668727
'Sperm capacitation in vitro is thought to be correlated with the increased protein tyrosine phosphorylation of a subset of sperm components. Our group recently used a pharmacological approach to demonstrate that calmodulin (CaM), a 17 kDa calcium sensor protein, has a role in sperm capacitation. In the present study, we ... More
Automated image analysis of live/dead staining of the fungus Aureobasidium pullulans on microscope slides and leaf surfaces.
Authors:Nelson CD, Spear RN, Andrews JH
Journal:Biotechniques
PubMed ID:11056819
'An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue, while nonviable cells stained with DEAD Red. Image analysis macro programs ... More
A2E-epoxides damage DNA in retinal pigment epithelial cells. Vitamin E and other antioxidants inhibit A2E-epoxide formation.
Authors:Sparrow JR, Vollmer-Snarr HR, Zhou J, Jang YP, Jockusch S, Itagaki Y, Nakanishi K
Journal:J Biol Chem
PubMed ID:12646558
'The autofluorescent pigments that accumulate in retinal pigment epithelial cells with aging and in some retinal disorders have been implicated in the etiology of macular degeneration. The major constituent is the fluorophore A2E, a pyridinium bisretinoid. Light-exposed A2E-laden retinal pigment epithelium exhibits a propensity for apoptosis with light in the ... More