ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered

Citations & References (38)

Invitrogen™

ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered

加水分解の際、ELF™ 97ホスファターゼ基質は酵素活性部位で明るく光安定性のある黄緑色蛍光沈殿物を生じさせます。この蛍光沈殿物には、内因性蛍光と容易に区別できる非常に大きなストークスシフトなど、いくつかの独自のスペクトル特性があります。詳細を見る

製品番号（カタログ番号）	数量
E6588	1 mL

製品番号（カタログ番号） E6588

価格（JPY）

108,600

お問い合わせください ›

加水分解の際、ELF™ 97ホスファターゼ基質は酵素活性部位で明るく光安定性のある黄緑色蛍光沈殿物を生じさせます。この蛍光沈殿物には、内因性蛍光と容易に区別できる非常に大きなストークスシフトなど、いくつかの独自のスペクトル特性があります。

研究用にのみ使用できます。診断用には使用いただけません。

細胞透過性細胞透過性

濃度5 mM

励起／発光345⁄530

使用対象 (装置)蛍光顕微鏡, マイクロアレイ, フローサイトメーター

標識または色素ELF 97

製品ラインELF

数量1 mL

研究カテゴリー分化

出荷条件室温

基質ホスファターゼ基質

検出法蛍光

Substrate Properties化学基質

Target Enzymeホスファターゼ

Unit SizeEach

組成および保存条件

フリーザー（-5～-30度）に保存し、遮光してください。

よくあるご質問（FAQ）

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (38)

引用および参考文献

Abstract

Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.

Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM

Journal:Stem cells (Dayton, Ohio)

PubMed ID:16293579

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM

Journal:Stem Cells

PubMed ID:17284651

'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More

Quantitative differences in phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes.

Authors:Ekins S, Murray GI, Burke MD, Williams JA, Marchant NC, Hawksworth GM

Journal:Drug Metab Dispos

PubMed ID:8591730

'Testosterone (250 microM), 7-ethoxycoumarin (25 microM), and 1-chloro-2,4-dinitrobenzene (CDNB, 50 microM) were used as substrates to compare phase I and II metabolism in rat precision-cut liver slices and rat hepatocytes. Overall clearance to metabolites was significantly greater in hepatocytes for testosterone (1.9- to 16.9-fold), 7-ethoxycoumarin (O-deethylation, 14.8-fold; glucuronidation, 3.1-fold), and ... More

Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1.

Authors:Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ

Journal:Clin Chim Acta

PubMed ID:15748605

'OBJECTIVE: As alkaline phosphatase may play a role in cell differentiation, our aim was to study the possible role of this enzyme in the differentiation of preadipocytes (3T3-L1 cells) into adipocytes. RESEARCH METHODS AND PROCEDURES: 3T3-L1 cells were grown in medium containing insulin, dexamethasone and IBMX to induce adipogenesis. Adipogenesis ... More

Fluorescence in situ hybridization of scarce leptin receptor mRNA using the enzyme-labeled fluorescent substrate method and tyramide signal amplification.

Authors:Breininger JF, Baskin DG

Journal:J Histochem Cytochem

PubMed ID:11101627

'To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification (TSA) and Enzyme-Labeled Fluorescent alkaline phosphatase substrate (ELF) methods. First, ... More

View all ELF™ 97 Phosphatase Substrate (ELF™ 97 Phosphate), 0.2 μm Filtered citations