ELF™ 97 Endogenous Phosphatase Detection Kit

Citations & References (32)

Invitrogen™

ELF™ 97 Endogenous Phosphatase Detection Kit

ELF™ 97 Endogenous Phosphatase Kit™は、酵素活性部位で加水分解により明るく光安定性のある黄緑色蛍光沈殿を生じる、ELF 97ホスファターゼ基質を用いてホスファターゼ活性を検出するために使用できます。この蛍光沈殿物には、内因性蛍光と容易に区別できる180詳細を見る

製品番号（カタログ番号）	数量
E6601	1 kit

製品番号（カタログ番号） E6601

価格（JPY）

96,600

お問い合わせください ›

1 kit

ELF™ 97 Endogenous Phosphatase Kit™は、酵素活性部位で加水分解により明るく光安定性のある黄緑色蛍光沈殿を生じる、ELF 97ホスファターゼ基質を用いてホスファターゼ活性を検出するために使用できます。この蛍光沈殿物には、内因性蛍光と容易に区別できる180 nmの非常に大きなストークスシフトなど、いくつかの独自のスペクトル特性があります。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

染色剤タイプELF 97

数量1 kit

出荷条件湿氷

基質ELF™ 97リン酸

基質特性化学基質

基質タイプホスファターゼ基質

標的酵素ホスファターゼ

Emission345⁄530

使用対象（アプリケーション）ホスファターゼ検出

使用対象 (装置)蛍光顕微鏡

製品ラインELF

製品タイプホスファターゼアッセイ

Unit SizeEach

組成および保存条件

冷蔵庫（2℃～8℃）に保存し、遮光してください。

よくあるご質問（FAQ）

Can the ELF 97 reagent be applied to live cells?

ELF 97 substrate is cell impermeant. It may be used on live cells that exhibit phosphatase activity on the surface of the cells, but not intracellular phosphatase activity in live cells. The alternative product for live-cell detection of phosphatase activity is the cell-permeable Alkaline Phosphatase Live Stain (Cat. No. A14353).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can ELF 97 stained cell/tissue samples be multiplexed with antibody labeling?

No. The ELF 97 reagent does not covalently attach to any cellular components and may be washed away with any subsequent antibody labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (32)

引用および参考文献

Abstract

Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.

Authors:Plaia TW,Josephson R,Liu Y,Zeng X,Ording C,Toumadje A,Brimble SN,Sherrer ES,Uhl EW,Freed WJ,Schulz TC,Maitra A,Rao MS,Auerbach JM

Journal:Stem cells (Dayton, Ohio)

PubMed ID:16293579

Primitive adult hematopoietic stem cells can function as osteoblast precursors.

Authors:Olmsted-Davis EA, Gugala Z, Camargo F, Gannon FH, Jackson K, Kienstra KA, Shine HD, Lindsey RW, Hirschi KK, Goodell MA, Brenner MK, Davis AR

Journal:Proc Natl Acad Sci U S A

PubMed ID:14673088

'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here ... More

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

Authors:Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM

Journal:Stem Cells

PubMed ID:17284651

'As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line''s characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of ... More

Osteoclast responses to lipopolysaccharide, parathyroid hormone and bisphosphonates in neonatal murine calvaria analyzed by laser scanning confocal microscopy.

Authors:Suzuki K, Takeyama S, Kikuchi T, Yamada S, Sodek J, Shinoda H

Journal:J Histochem Cytochem

PubMed ID:16087705

'Because the development and activity of osteoclasts in bone remodeling is critically dependent on cell-cell and cell-matrix interactions, we used laser confocal microscopy to study the response of osteoclasts to lipopolysaccharide (LPS; 10 microg/ml), parathyroid hormone (PTH; 10(-8) M), and bisphosphonates (BPs; 1-25 microM clodronate or 0.1-2.5 microM risedronate) in ... More

Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols.

Authors:Filgueira L

Journal:J Histochem Cytochem

PubMed ID:14966208

Osteoclasts are the only bone-resorbing cells. In addition to other specific properties, osteoclasts are characterized by their expression of tartrate-resistant acidic phosphatase (TRAP), which is usually detected using a histochemical method for light microscopy. Using ELF97 phosphatase substrate, this study describes a new fluorescence-based method for TRAP detection. The fluorescence-based ... More

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