EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

Citations & References (9)

Invitrogen™

EnzChek™ Protease Assay Kit, red fluorescence, 100-1000 assays

製品番号（カタログ番号）	数量
E6639	1 Kit

製品番号（カタログ番号） E6639

価格（JPY）

58,400

온라인 행사

Ends: 27-Mar-2026

97,400

割引額 39,000 (40%)

お問い合わせください ›

1 Kit

EnzChek™プロテアーゼアッセイキット、赤色蛍光は、メタロプロテアーゼ、セリンプロテアーゼ、酸プロテアーゼ、およびスルフヒドリルプロテアーゼを検出するための高速でシンプルな直接蛍光ベースのアッセイです。プロテアーゼ活性に比例して発生する蛍光強度の増加は、蛍光マイクロプレートリーダーを使用して測定できます。

蛍光マイクロプレートアッセイの全製品ラインアップをご覧ください。

•一般化されたメカニズムにより、さまざまなプロテアーゼを測定できます
• プロテアーゼ動態の連続測定に使用できます
• 励起波長／発光波長はTexas Red™色素（589/617 nm）と同様です

このEnzChek™プロテアーゼアッセイキットには、カゼイン誘導体が含まれています。この誘導体は、蛍光色素の消光を引き起こす赤色蛍光のBODIPY™ TR-X色素で十分に標識されています。プロテアーゼ触媒作用による加水分解により、蛍光強度の高いBODIPY™ TR-X色素標識ペプチドが放出され、溶液中のプロテアーゼ活性の定量的検出が可能になります。赤色蛍光のBODIPY™ TR-X色素は、Texas Red™フルオロフォアと同様の励起および発光スペクトルを持ちます。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光強度

数量1 Kit

出荷条件室温

基質特性タンパク質ベースの基質

基質タイププロテアーゼ基質

標的酵素プロテアーゼ

使用対象（アプリケーション）プロテアーゼアッセイ

使用対象 (装置)蛍光マイクロプレートリーダー

製品ラインEnzChek

製品タイププロテアーゼアッセイ

Unit SizeEach

組成および保存条件

フリーザー（-5℃～-30℃）に保存し、遮光してください。

よくあるご質問（FAQ）

What is the optimal pH range for protease activity detection using the EnzChek Protease Assay Kit?

The digestion buffer provided in the kit (pH 7.8) is recommended for detecting the protease activity of most proteolytic enzymes with activity optima from pH 7.4 to 8.0. However, if you are working with an enzyme that requires activation compounds or a unique pH environment, then prepare the specific buffer required in place of the digestion buffer.

What is the shelf life for EnzChek Protease Assay kits (Cat. Nos. E6638, E6639)?

As stated in the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp06638.pdf) on page 2 under section "Storage and Handling", these kits are stable for 6-12 months if stored as indicated.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long is the reconstituted BODIPY substrate in the EnzChek Protease Assay Kit, red fluorescence, 100-1000 assays (Cat. No. E6639) stable?

Reconstituted BODIPY casein substrates may be stored at 4 degrees C for 2-4 weeks. We recommend the addition of sodium azide at a final concentration of 2 mM to act as a preservative. If longer storage is required, freeze at -20 degrees C. Protect from light and avoid repeated freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (9)

引用および参考文献

Abstract

Quenched BODIPY dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement.

Authors:Jones LJ, Upson RH, Haugland RP, Panchuk-Voloshina N, Zhou M, Haugland RP

Journal:Anal Biochem

PubMed ID:9299009

'We have prepared casein conjugates of two BODIPY dyes for use as fluorogenic protease substrates in homogeneous assays. Both conjugates are labeled to such an extent that the dyes are efficiently quenched in the protein, yielding virtually nonfluorescent substrate molecules. These fluorogenic substrates release highly fluorescent BODIPY dye-labeled peptides upon ... More

Subnanoliter enzymatic assays on microarrays.

Authors:Angenendt P, Lehrach H, Kreutzberger J, Glökler J

Journal:Proteomics

PubMed ID:15700241

'Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation of these assays. However, the final transfer of enzymatic assays ... More

Continuous assay of proteases using a microtiter plate fluorescence reader.

Authors:Menges DA, Ternullo DL, Tan-Wilson AL, Gal S

Journal:Anal Biochem

PubMed ID:9398357

A protease-dependent mechanism for initiating T-dependent B cell responses to large particulate antigens.

Authors:Catron DM, Pape KA, Fife BT, van Rooijen N, Jenkins MK,

Journal:J Immunol

PubMed ID:20208013

Ab production is critical for antimicrobial immunity, and the initial step in this process is the binding of Ag to the BCR. It has been shown that small soluble proteins can directly access the lymph node follicles to reach naive B cells, but virus particles must be translocated into follicles ... More

Selective modulation of superantigen-induced responses by streptococcal cysteine protease.

Authors:Kansal RG, Nizet V, Jeng A, Chuang WJ, Kotb M

Journal:J Infect Dis

PubMed ID:12552423

Streptococcal pyrogenic exotoxin (Spe) B, a streptococcal cysteine protease, is believed to be important in group A streptococcal (GAS) pathogenesis. The present study examined the effect of SpeB on the activity of superantigenic exotoxins secreted by M1T1 GAS isolates. The proliferative response of human lymphocytes to culture supernatant (SUP) from ... More

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