Invitrogen™

Novex™ TBE-Urea Gels, 10%

Name: Novex™ TBE-Urea Gels, 10%
SKU: EC6875BOX

Novex™ TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands.

テクニカルサポートオーダーサポート

表示を変更

製品番号（カタログ番号）	ウェル
EC6875BOX	10ウェル
EC68752BOX	12ウェル
EC68755BOX	15ウェル

3 オプション

製品番号（カタログ番号） EC6875BOX

価格（JPY）

44,400

Each

ウェル:

10ウェル

一括またはカスタム形式をリクエストする

Novex™ TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands. These 10% gels are optimized for the analysis and purification of products ranging from 20–55 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and northern blot analysis.

Novex TBE-Urea Gels can be stained by silver staining, ethidium bromide, and SYBR™ Green staining techniques after electrophoresis. The compact gels are available in 6%, 10%, or 15% formats and are designed to run on the XCell SureLock™ Mini-Cell.

Advantages of TBE-Urea Gels:

Contains 7M urea for maximum denaturation
Excellent resolution for fast size and purity confirmations of DNA or RNA oligos
High resolution of short single-strand oligonucleotides
Requires ∼10% sample concentration and volume of large or agarose gels
Accurate and reproducible results

Formulation

Novex TBE-Urea gels are made with high-purity reagents, including Tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, APS, and 7M urea. Strict quality control ensures that gels and buffers are DNase- and RNase-free.

Recommended Buffers

For optimum performance, Novex™ TBE-Urea sample buffers and Novex™ TBE Running Buffer are strongly recommended for use with these gels. For analytical applications, Novex TBE-Urea Sample Buffer is recommended contains urea, the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.

For Research Use Only. Not for use in diagnostic procedures.

仕様

概要Novex TBE-Urea Gels, 10%, 10-well

使用対象 (装置)XCell SureLock Mini-Cell

数量10ゲル／箱

サンプルタイプSingle-stranded DNA Oligos/RNA

出荷条件湿氷

ゲル濃度10%

ゲルタイプTBE-尿素

製品タイプTBEゲル

分離範囲20 to 55 bp

ウェル10ウェル

Unit SizeEach

組成および保存条件

ゲルのカタログ番号は、1箱分を参照しています。1箱に10つのゲルが入っています。Novex™ TBE-尿素ゲルの有効期限は、ゲルの種類によって4～8週間です。4℃で保存。

よくあるご質問（FAQ）

Applied Biosystems® real-time PCRシステムでマルチプレックス反応は可能ですか。また、何種類まで可能でしょうか。

Applied Biosystems® real-time PCRシステムではマルチプレックスアッセイに対応しています。現在弊社では通常1つのwellで2色までの蛍光色素の使用をサポートしております。また、2色以上の蛍光色素を1つのwellで同時に検出することも可能です。ただ、そのためにはプライマー同士の競合反応を抑えるようにプライマー濃度の最適化をすることが必須です。

I am getting high background and smeared bands on my TBE-urea gels. What should I do?

Here are some suggestions for your experiments:

- The RNA samples may have been degraded by RNases. Use standard precautions to prevent contamination.
- Make sure samples are heated for 3 minutes at 70°C just prior to loading. If this is not done, the oligonucleotides will not be fully denatured, which may result in a smeared background.
- Be sure to vigorously flush urea out of sample wells just prior to loading the sample. Urea will continually seep from the gel into the well. Urea is very dense and will force the sample into a ball.
- A sample volume over 10 µL may result in smearing. This volume is less than may be used with agarose gels.
- If ultrapure water (18 milliohms) is not used, smeared bands and high background may result.
- Check to make sure the running and sample buffers have been prepared and diluted correctly.
- Make sure to run the bromophenol blue until it reaches the slot. Stopping the run short can result in less than optimal results.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I use a sample buffer containing formamide with the Invitrogen TBE-urea system?

There are many sample buffer formulations used; however, we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide provided fuzzy, indistinct bands.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I stain my TBE gel or my TBE-urea gel? How?

Yes, for ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I am planning to use a TBE polyacrylamide gel for electrophoresis of my nucleic acid fragment. How do I know if the size of my fragment is suitable for a particular gel you offer?

To help you choose the TBE polyacrylamide gel that will provide optimal separation results for your nucleic acid fragment, we have designed gel migration charts for each gel type offered. Please check the TBE gel migration charts on page 74 of the Novex Pre-Cast Gel Electrophoresis Guide (https://tools.thermofisher.com/content/sfs/manuals/electrophoresisguide_man.pdf).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all Novex™ TBE-Urea Gels, 10% FAQs