β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
Invitrogen™

β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

β-hydroxybutyrate (β-HB), C4H8O3, accounts for about 75% of the total ketone body. Patients with diabetic ketoacidosis have increased production of NADH, which promotes the conversion of acetoacetic acid into β-HB. Therefore, the level of β-hydroxybutyrate can be used as an index to evaluate glycosuria.
製品番号(カタログ番号)数量
EEA04696 Tests
製品番号(カタログ番号) EEA046
価格(JPY)
112,400
Each
数量:
96 Tests

With this kit, β-hydroxybutyrate dehydrogenase catalyzes the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfers electrons to WST-8 to produce a yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm. This kit is useful for researchers studying ketone body metabolism and diabetes.

Key features

  • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
  • Broad range of kits across key analytes and targets within the most important metabolic pathways
  • Consistent—strict quality control criteria to ensure reproducible and reliable results
For Research Use Only.
仕様
アッセイ範囲0.01 to 2.00 mmol/L
アッセイ感度0.01 mmol/L
使用対象 (装置)Microtiter Plate Reader
インキュベーション時間40 min.
アッセイ間のCV4.0%
アッセイ内CV2.0%
製品タイプKit
数量96 Tests
サンプル量10 μL
出荷条件Dry Ice, 2°C to 8°C
指名標的キットβ-Hydroxybutyrate (Ketone Body)
総アッセイ時間60 min.
検出法Colorimetric
イムノアッセイキットのフォーマットColorimetric Detection Kit
サンプルタイプSerum, Plasma, Urine, Animal Tissue
ターゲット種Universal
Unit SizeEach
組成および保存条件
Store at 2°C to 8°C.