Taq DNA Polymerase, native (5 U/μL)
Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific™

Taq DNA Polymerase, native (5 U/μL)

Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
テクニカルサポートオーダーサポート
表示を変更buttonViewtableView
製品番号（カタログ番号）フォーマット
EP0072With BSA
EP0282Without BSA
製品番号（カタログ番号） EP0072
価格（JPY）
22,400
온라인 행사
Ends: 27-Mar-2026
37,400
割引額 15,000 (40%)
Each
お問い合わせください ›
フォーマット:
With BSA
一括またはカスタム形式をリクエストする
Thermo Scientific Taq DNA Polymerase, native, is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Available with or without the stabilizing agent BSA, Taq DNA Polymerase, native, with BSA, is often considered the best choice when amplifying DNA samples of lower purity, e.g., genomic DNA from mouse tail.

Both native Taq DNA polymerases are supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at a wide range of magnesium concentrations and decreases unspecific priming

Features

  • Thermostable with half-life more than 40 min at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • DNA labeling
  • High throughput PCR

Notes

  • The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
  • The 10X Taq Buffer without Detergent is recommended for microarray experiments.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
フォーマットWith BSA
最適反応温度72℃
ポリメラーゼTaq DNAポリメラーゼ
製品タイプDNAポリメラーゼ
数量300 U
研究カテゴリーPCR
出荷条件ドライアイス
濃度5 U/μL
使用対象（アプリケーション）Standard PCR
Unit SizeEach
組成および保存条件
内容：
• 100 µL Taq DNAポリメラーゼ、天然型（5 U/µL）
• 2x1.25 mL 10倍Taqバッファー、KCl含有
• 2x1.25 mL 10倍Taqバッファー、（NH4）2SO4含有
• 2x1.25 mL 25 mM MgCl2
• 0.25 mL 20 mg/mL BSA

-20℃で保存してください。