phi29 DNA Polymerase (10 U/μL)

The error rate of phi29 DNA Polymerase is 1 x 10^-5.
The error rate of phi29 DNA Polymerase was measured according to the method described in literature:
Mielinis, P., Sukackaitė, R., Serapinaitė, A., Samoilovas, F., Alzbutas, G., Matjošaitis, K., & Lubys, A. (2021). MUA-based molecular indexing for rare mutation detection by Next-Generation sequencing. Journal of Molecular Biology, 433(19), 167209. https://doi.org/10.1016/j.jmb.2021.167209

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phi29 DNA Polymerase should be able to incorporate 5-methyl-dCTP nucleotides and other modified nucleotides.
The incorporation of modified nucleotides by phi29 DNA Polymerase can be reviewed in the literature:
Jakubovska, J., Tauraitė, D., Birštonas, L., & Meškys, R. (2018). N 4-acyl-2′-deoxycytidine-5′-triphosphates for the enzymatic synthesis of modified DNA. Nucleic Acids Research, 46(12), 5911–5923. https://doi.org/10.1093/nar/gky435

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The optimal reaction time for DNA amplification with phi29 DNA Polymerase is 4 hours. For samples with ≥1 pg of DNA input, DNA amplification time can be shortened to 2 hours if maximizing product yield is not essential.

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Yes. phi29 DNA Polymerase can work with different types of sample input such as purified DNA, liquid media culture, agar plate colonies, etc.

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Yes, we recommend heat-inactivating phi29 DNA Polymerase at 65 degrees C for 10 min.

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