Are there robotic scripts for running E-Gel 48/96 or E-PAGE 48/96 gels?
Robotic scripts for running E-Gel 96 Agarose Gels and E-PAGE 96 Gels on Beckman Coulter's Biomek FX workstation can be found on our website at: www.thermofisher.com/egels (click the Labware Definitions link in the left navigation pane).
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
What is the difference between the EG and EP program for the E-Base system?
The EG program is to run E-Gel 96 and 48 gels, while the EP is to run the E-PAGE 96 and 48 gels.
Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.
What transfer buffer do I use to blot E-PAGE gels in a semi-wet transfer protocol (with the XCell II blot module)?
NuPAGE Transfer Buffer with 10% methanol provides optimal transfer of E-PAGE gels in the XCell II Blot Module. The NuPAGE Antioxidant is used in the transfer buffer for blotting reduced proteins and prevents the proteins from re-oxidizing. The Tris-Glycine Transfer Buffer has not been tested and it is not known as to what the transfer efficiencies would be like.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
What are some troubleshooting hints if there is high background after Coomassie staining of E-PAGE?
If you observe high background after Coomassie staining/destaining, here are some things to check:
It is very important to use 0.015% Coomassie R-250. Even using 0.03% will make Coomassie destaining more difficult.
The gel shouldn't be left in stain for more than 1 hour.
Increasing the destain time may reduce background.
Warming the gel and destain solution is also very important for best results.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
What is the function of the E-Editor 2.0 software?
The E-Editor 2.0 software allows you to quickly reconfigure digital images of E-Gel 48, E-Gel 96, E-PAGE 48 and E-PAGE 96 gel results for analysis and documentation. You can capture an image of the gel and then use the E-Editor 2.0 software to:
-Align and arrange the lanes in the image to any 48, 96, or 384 image
-Save the reconfigured image for further analysis
-Copy and paste selected lanes or the entire image into other applications for printing, saving, e-mailing, and/or publishing on the Web.
The E-Editor 2.0 does not take perform densitometry analysis from your gel images. The E-Editor 2.0 can be downloaded for free.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.