E-PAGE™ Midi Protein Gels, 3.7 mm

Robotic scripts for running E-Gel 96 Agarose Gels and E-PAGE 96 Gels on Beckman Coulter's Biomek FX workstation can be found on our website at: www.thermofisher.com/egels (click the Labware Definitions link in the left navigation pane).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

The EG program is to run E-Gel 96 and 48 gels, while the EP is to run the E-PAGE 96 and 48 gels.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

For SDS-PAGE and staining or blotting, we recommend using the 4X E-PAGE Loading Buffer 1 for preparing samples. We do not recommend using any other SDS-PAGE sample buffer. The E-PAGE Loading Buffer 1 is optimized for E-PAGE gels. If the sample is already in NuPAGE LDS sample buffer or the Tris-Glycine SDS sample buffer, it is fine to run on the E-PAGE gels. However, there may be a loss of resolution in the bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The E-Editor 2.0 Software can be downloaded for free from the Thermo Fisher Scientific website (search for 'e-editor 2.0').

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Due to the thickness of the E-PAGE gels, they are not compatible with the iBlot3 device for transfer. Recommended methods for transfer of E-PAGE gels are semi-dry blotting or semi-wet blotting in the XCell II Blot Module. Methods are described in the E-PAGE Technical Guide.

Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.