ProcartaPlex™ Human Immune Response Panel, 80plex

The ProcartaPlex Human Immune Response Panel 80plex enables the study of immune function by analyzing 80 protein targets in a single well using Luminex xMAP technology. Analyze 80 cytokine, chemokine, and growth factor targets simultaneously for efficient immune response profiling, biomarker discovery, and validation. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup.

ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.This kit is not compatible with the MAGPIX instrument.

Target [bead region]

• Cytokines: G-CSF [42], GM-CSF [44], IFN alpha [48], IFN gamma [43], IL-10 [28], IL-12p70 [34], IL-13 [35], IL-15 [65], IL-16 [70], IL-17A [36], IL-18 [66], IL-1 alpha [62], IL-1 beta [18], IL-2 [19], IL-20 [81], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14], IL-3 [73], IL-31 [37], IL-34 [79], IL-37 [7], IL-4 [20], IL-5 [21], IL-6 [25], IL-7 [26], IL-8 [27], IL-9 [52], LIF [15], M-CSF [67], MIF [53], TNF beta [54], TNF alpha [45], TSLP [80]
• Chemokines: BLC [29], CCL1 (I-309) [83], CCL17 (TARC) [91], CCL21 (6Ckine/SLC) [96], CCL23 (MPIF) [98], CCL25 (TECK) [94], CXCL6 (GCP-2) [99], ENA-78 [82], Eotaxin [33], Eotaxin-2 [30], Eotaxin-3 [49], Fractalkine (CX3CL1) [59], Gro-alpha (CXCL1) [61], IP-10 (CXCL10) [22], I-TAC (CXCL11) [57], MCP-1 (CCL2) [51], MCP-2 (CCL8) [8], MCP-3 (CCL7) [68], MCP-4 (CCL13) [90], MDC (CCL22) [87], MIG (CXCL9) [69], MIP-1 alpha (CCL3) [12], MIP-1 beta (CCL4) [47], MIP-2 alpha (CXCL2) [95], MIP-3 beta (CCL19) [93], MIP-3 alpha (CCL20) [56]
• Growth factors: NGF beta [55], FGF-2 [75], HGF [46], MMP-1 [64], SCF [39], VEGF-A [78]
• Soluble receptors: APRIL [88], BAFF [86], CD30 [84], CD40L (CD154) [74], IL-2R (CD25) [9], TNF-RII [85], TRAIL [58], TREM-1 [13], TWEAK [97]
• Serine proteases: Granzyme A [38], Granzyme B [77]
• Lectin pentraxin: Gal-3 [89], PTX3 [100]

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

QuantiGene RNA testing—one machine, two applications

Gain deeper insight with complementary and highly correlative gene expression and protein assays. With the largest multiplex panel for measurement of protein and RNA, you can now profile more biomarkers with less sample. The Immune Response 80-Plex Human QuantiGene Plex Panel (Cat. No. QGP-180-10080) enables efficient immune response profiling and biomarker discovery.