What species can ProcartaPlex Platinum Assay Buffer for Plasma Samples be used with?
ProcartaPlex Platinum Assay Buffer for Plasma Samples is designed to be used with the ProcartaPlex Platinum assay format. Currently, this format has been validated for human samples only. Other species have not been tested.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
What kits can ProcartaPlex Platinum Assay Buffer for Plasma Samples be used with?
ProcartaPlex Platinum Assay Buffer for Plasma Samples is designed to be used with the ProcartaPlex Platinum assay format. It is used for dilution of plasma samples and gives spike- and dilution-recovery results in the range of 70-130%. ProcartaPlex Platinum Assay Buffer for Plasma Samples is included in our Platinum ProcartaPlex Human Basic Kit for Plasma Samples (Cat.No. EPXP010-10421-901) and off-the-shelf Platinum panels. It is also offered as a stand-alone item (Cat. No. EPXP-11112-000).
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
I am getting poor recovery with my ProcartaPlex multiplex immunoassay. What can I do?
Here are possible causes and solutions:
- The wrong cell culture media may have been used to prepare the standards. We recommend using the same cell culture media that was used to culture the cells.
- The samples and antigen standards may not have been stored on ice. We recommend preparing the samples and standards on ice before setting up the assay.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
I am seeing low signal or sensitivity in my ProcartaPlex multiplex immunoassay. What should I do?
Standards may not have been reconstituted and diluted correctly. We recommend preparing fresh antigen standards following the instructions provided in the corresponding ProcartaPlex user guide.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
With my ProcartaPlex multiplex immunoassay, I am getting a low bead count. What is causing this?
Here are possible causes and solutions:
- Volume of the bead solution is too low. We recommend adding 120 µL Reading Buffer into each well and shaking at 600 rpm for 5 mins at room temperature to resuspend beads before reading on the Luminex instrument.
- High bead aggregation. We recommend vortexing the bead suspension well before using in the assay and ensuring that the beads are properly mixed during the incubation steps.
- Dyes contained in the beads are photo-bleached from overexposure to light. We recommend storing the bead solution and the 96-well plate in the dark.
- Samples causing the instrument to clog. We recommend removing the 96-well Flat Bottom Plate and performing a wash and rinse to the instrument and then re-running the assay with further dilution of samples.
- Probe height is incorrect. We recommend referring to the Luminex manual for proper adjustment of the needle height.
- The instrument needle is partially clogged. We recommend replacing or cleaning the needle according to the manufacturer's recommendations.
- Beads stuck to the bottom of the plate. We recommend confirming that the plate shaker is set to 600 rpm and shaking for at least 5 mins before reading.
- Air bubble in the sample loop. We recommend referring to the specific Luminex manual for your assay for proper removal of the air bubble.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
