FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

Citations & References (4)

Invitrogen™

FilmTracer™ FM™ 1-43 Green Biofilm Cell Stain

FM色素は脂溶性スニル化合物で、原形質膜や小水疱形成などのさまざまな研究で使用されています。水溶性FM™色素は、水性媒体では実質的に蛍光を示さず、表面膜に挿入されると強い蛍光を発するといわれています。FM™ 1-43染色剤は、緑膿菌、大腸菌、ブドウ球菌属詳細を見る

製品番号（カタログ番号）	数量
F10317	各 1

製品番号（カタログ番号） F10317

価格（JPY）

76,100

お問い合わせください ›

FM色素は脂溶性スニル化合物で、原形質膜や小水疱形成などのさまざまな研究で使用されています。水溶性FM™色素は、水性媒体では実質的に蛍光を示さず、表面膜に挿入されると強い蛍光を発するといわれています。FM™ 1-43染色剤は、緑膿菌、大腸菌、ブドウ球菌属、Acidothiobacillus caldus、ビブリオ属コレラエなどの複合バイオフィルム群に特異的に細胞体を染色するために使用されています。

研究用にのみ使用できます。診断用には使用いただけません。

色Green

検出法蛍光

染色剤タイプFM 1-43⁄脂質

励起波長域472⁄580

使用対象 (装置)共焦点顕微鏡, 蛍光顕微鏡, フローサイトメトリー, マイクロプレートリーダー, 蛍光イメージャー

製品ラインFilmTracer、Molecular Probes

数量各 1

出荷条件室温

標識タイプその他のラベルまたは色素

製品タイプ色素

SubCellular Localization細胞膜&脂質, Lipids

Unit SizeEach

組成および保存条件

室温で保存し、乾燥下で遮光します。

よくあるご質問（FAQ）

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (4)

引用および参考文献

Abstract

A novel role for enzyme I of the Vibrio cholerae phosphoenolpyruvate phosphotransferase system in regulation of growth in a biofilm.

Authors:Houot L, Watnick PI,

Journal:J Bacteriol

PubMed ID:17981973

Glucose is a universal energy source and a potent inducer of surface colonization for many microbial species. Highly efficient sugar assimilation pathways ensure successful competition for this preferred carbon source. One such pathway is the phosphoenolpyruvate phosphotransferase system (PTS), a multicomponent sugar transport system that phosphorylates the sugar as it ... More

Fluorescence staining of live cyanobacterial cells suggest non-stringent chromosome segregation and absence of a connection between cytoplasmic and thylakoid membranes.

Authors:Schneider D, Fuhrmann E, Scholz I, Hess WR, Graumann PL,

Journal:BMC Cell Biol

PubMed ID:17767716

BACKGROUND: In spite of their abundance and importance, little is known about cyanobacterial cell biology and their cell cycle. During each cell cycle, chromosomes must be separated into future daughter cells, i.e. into both cell halves, which in many bacteria is achieved by an active machinery that operates during DNA ... More

Genome Sequence and Composition of a Tolyporphin-Producing Cyanobacterium-Microbial Community.

Authors:

Journal:Appl Environ Microbiol

PubMed ID:28754701

Beta-Defensin 2 and 3 Promote Bacterial Clearance of Pseudomonas aeruginosa by Inhibiting Macrophage Autophagy through Downregulation of Early Growth Response Gene-1 and c-FOS.

Authors:

Journal:Front Immunol

PubMed ID:29487594