Fluo-4 Direct™ Calcium Assay Kit

Citations & References (8)

Invitrogen™

Fluo-4 Direct™ Calcium Assay Kit

Name: Fluo-4 Direct™ Calcium Assay Kit
SKU: F10472

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製品番号（カタログ番号）	数量
F10472	100 ml
F10471	100 ml
F10473	1000 ml

3 オプション

製品番号（カタログ番号） F10472

価格（JPY）

103,200

Each

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数量:

100 ml

Fluo-4 Direct™カルシウムアッセイキットは、次のような均一な蛍光カルシウムアッセイを提供するように調製されています。
1.細胞への取り込みが容易
2.大きなアッセイウィンドウを実現
3.特定の細胞性蛍光にほとんどまたはまったく影響を与えずに、完全培地でカルシウムインジケーターから発生するバックグラウンド蛍光を抑制

この高度な組成により、血清を含む培地の存在下で、アッセイをシンプルな「添加のみ」のフォーマットで実行できます。Fluo-4 Direct™は、カルシウム検出試薬のFluo-4ファミリーに3番目に加わった製品です。Fluo-4 AMとFluo-4 NWはどちらもアッセイ検出前に培地を除去する必要がありますが、Fluo-4 NWでは、細胞の取り込みを容易に行うためにPowerLoad™試薬を使用することで利便性が向上します。Fluo-4 Direct™カルシウムアッセイキットは、細胞の取り込みを容易に行うためにPowerLoad™を使用して調製されている点で、Fluo-4 NWと似ています。ただし、完全培養培地の存在下で使用でき、アッセイで生成される特定の細胞蛍光を損なうことなくバックグラウンド蛍光を効率的に抑制できるという点で、独自性があります。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

検出法蛍光

染色剤タイプ蛍光色素ベース

形状粉末

修飾化学的

数量100 ml

出荷条件室温

細胞内局在核、オルガネラ、細胞質

色Green

Emission可視

使用対象（アプリケーション）カルシウムアッセイ

使用対象 (装置)共焦点顕微鏡, 蛍光顕微鏡, ハイコンテント装置, HTSリーダー, マイクロプレートリーダー, 蛍光イメージャー

製品ラインMolecular Probes

製品タイプカルシウムアッセイキット

Unit SizeEach

組成および保存条件

100 ml Fluo-4 Direct™カルシウムアッセイ試薬（コンポーネントA）
2 × 77 mgプロベネシド（コンポーネントB）
200 ml Fluo-4 Direct™カルシウムアッセイバッファー（コンポーネントC）
≦-20℃で保存してください。乾燥した状態で、遮光してください。

よくあるご質問（FAQ）

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (8)

引用および参考文献

Abstract

High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.

Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,

Journal:J R Soc Interface

PubMed ID:21490003

'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More

Transient receptor potential vanilloid-1 (TRPV1) is a mediator of lung toxicity for coal fly ash particulate material.

Authors:Deering-Rice CE, Johansen ME, Roberts JK, Thomas KC, Romero EG, Lee J, Yost GS, Veranth JM, Reilly CA,

Journal:Mol Pharmacol

PubMed ID:22155782

'Environmental particulate matter (PM) pollutants adversely affect human health, but the molecular basis is poorly understood. The ion channel transient receptor potential vanilloid-1 (TRPV1) has been implicated as a sensor for environmental PM and a mediator of adverse events in the respiratory tract. The objectives of this study were to ... More

20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.

Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,

Journal:J Biol Chem

PubMed ID:22389490

'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More

Screening ß-arrestin recruitment for the identification of natural ligands for orphan G-protein-coupled receptors.

Authors:Southern C, Cook JM, Neetoo-Isseljee Z, Taylor DL, Kettleborough CA, Merritt A, Bassoni DL, Raab WJ, Quinn E, Wehrman TS, Davenport AP, Brown AJ, Green A, Wigglesworth MJ, Rees S,

Journal:J Biomol Screen

PubMed ID:23396314

A variety of G-protein-coupled receptor (GPCR) screening technologies have successfully partnered a number of GPCRs with their cognate ligands. GPCR-mediated ß-arrestin recruitment is now recognized as a distinct intracellular signaling pathway, and ligand-receptor interactions may show a bias toward ß-arrestin over classical GPCR signaling pathways. We hypothesized that the failure ... More

A novel in vitro model system for smooth muscle differentiation from human embryonic stem cell-derived mesenchymal cells.

Authors:Guo X, Stice SL, Boyd NL, Chen SY,

Journal:Am J Physiol Cell Physiol

PubMed ID:23220114

The objective of this study was to develop a novel in vitro model for smooth muscle cell (SMC) differentiation from human embryonic stem cell-derived mesenchymal cells (hES-MCs). We found that hES-MCs were differentiated to SMCs by transforming growth factor-ß (TGF-ß) in a dose- and time-dependent manner as demonstrated by the ... More

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