Fluo-4 Direct™ Calcium Assay Kit

Citations & References (8)

Invitrogen™

Fluo-4 Direct™ Calcium Assay Kit

Name: Fluo-4 Direct™ Calcium Assay Kit
SKU: F10473

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製品番号（カタログ番号）	数量
F10473	1000 ml
F10471	100 ml
F10472	100 ml

3 オプション

製品番号（カタログ番号） F10473

価格（JPY）

835,600

Each

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数量:

1000 ml

Fluo-4 Direct™カルシウムアッセイキットは、次のような均一な蛍光カルシウムアッセイを提供するように調製されています。
1.細胞への取り込みが容易
2.大きなアッセイウィンドウを実現
3.特定の細胞性蛍光にほとんどまたはまったく影響を与えずに、完全培地でカルシウムインジケーターから発生するバックグラウンド蛍光を抑制

この高度な組成により、血清を含む培地の存在下で、アッセイをシンプルな「添加のみ」のフォーマットで実行できます。Fluo-4 Direct™は、カルシウム検出試薬のFluo-4ファミリーに3番目に加わった製品です。Fluo-4 AMとFluo-4 NWはどちらもアッセイ検出前に培地を除去する必要がありますが、Fluo-4 NWでは、細胞の取り込みを容易に行うためにPowerLoad™試薬を使用することで利便性が向上します。Fluo-4 Direct™カルシウムアッセイキットは、細胞の取り込みを容易に行うためにPowerLoad™を使用して調製されている点で、Fluo-4 NWと似ています。ただし、完全培養培地の存在下で使用でき、アッセイで生成される特定の細胞蛍光を損なうことなくバックグラウンド蛍光を効率的に抑制できるという点で、独自性があります。

研究用にのみ使用できます。診断用には使用いただけません。

仕様

検出法蛍光

染色剤タイプ蛍光色素ベース

形状粉末

修飾化学的

数量1000 ml

出荷条件室温

細胞内局在核、オルガネラ、細胞質

色Green

Emission可視

使用対象（アプリケーション）細胞の生存率と増殖

使用対象 (装置)共焦点顕微鏡, 蛍光顕微鏡, ハイコンテント装置, HTSリーダー, マイクロプレートリーダー, 蛍光イメージャー

製品ラインMolecular Probes

製品タイプカルシウムアッセイキット

Unit SizeEach

組成および保存条件

1 L Fluo-4 Direct™カルシウムアッセイ試薬（コンポーネントA）
1.54 gプロベネシド（コンポーネントB）
≦-20℃で保存してください。乾燥した状態で、遮光してください。

よくあるご質問（FAQ）

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (8)

引用および参考文献

Abstract

High-resolution imaging of the immunological synapse and T-cell receptor microclustering through microfabricated substrates.

Authors:Biggs MJ, Milone MC, Santos LC, Gondarenko A, Wind SJ,

Journal:J R Soc Interface

PubMed ID:21490003

'T-cell activation via antigen presentation is associated with the formation of a macromolecular membrane assembly termed the immunological synapse (IS). The genesis of the IS and the onset of juxtacrine signalling is characterized by the formation of cell membrane microclusters and the organization of such into segregated microdomains. A central ... More

20-Hydroxyeicosatetraenoic acid (20-HETE) is a novel activator of transient receptor potential vanilloid 1 (TRPV1) channel.

Authors:Wen H, Östman J, Bubb KJ, Panayiotou C, Priestley JV, Baker MD, Ahluwalia A,

Journal:J Biol Chem

PubMed ID:22389490

'TRPV1 is a member of the transient receptor potential ion channel family and is gated by capsaicin, the pungent component of chili pepper. It is expressed predominantly in small diameter peripheral nerve fibers and is activated by noxious temperatures >42 °C. 20-Hydroxyeicosatetraenoic acid (20-HETE) is a cytochrome P-450 4A/4F-derived metabolite ... More

Selective Impairment of P2Y Signaling by Prostaglandin E2 in Macrophages: Implications for Ca2+-Dependent Responses.

Authors:Través PG, Pimentel-Santillana M, Carrasquero LM, Pérez-Sen R, Delicado EG, Luque A, Izquierdo M, Martín-Sanz P, Miras-Portugal MT, Boscá L,

Journal:J Immunol

PubMed ID:23479225

Extracellular nucleotides have been recognized as important modulators of inflammation via their action on specific pyrimidine receptors (P2). This regulation coexists with the temporal framework of proinflammatory and proresolution mediators released by the cells involved in the inflammatory response, including macrophages. Under proinflammatory conditions, the expression of cyclooxygenase-2 leads to ... More

Surfactant protein A integrates activation signal strength to differentially modulate T cell proliferation.

Authors:Mukherjee S, Giamberardino C, Thomas J, Evans K, Goto H, Ledford JG, Hsia B, Pastva AM, Wright JR,

Journal:J Immunol

PubMed ID:22219327

Pulmonary surfactant lipoproteins lower the surface tension at the alveolar-airway interface of the lung and participate in host defense. Previous studies reported that surfactant protein A (SP-A) inhibits lymphocyte proliferation. We hypothesized that SP-A-mediated modulation of T cell activation depends upon the strength, duration, and type of lymphocyte activating signals. ... More

HIV-1 Tat protein inhibits neurosecretion by binding to phosphatidylinositol 4,5-bisphosphate.

Authors:Tryoen-Tóth P, Chasserot-Golaz S, Tu A, Gherib P, Bader MF, Beaumelle B, Vitale N,

Journal:J Cell Sci

PubMed ID:23178941

HIV-1 transcriptional activator (Tat) enables viral transcription and is also actively released by infected cells. Extracellular Tat can enter uninfected cells and affect some cellular functions. Here, we examine the effects of Tat protein on the secretory activity of neuroendocrine cells. When added to the culture medium of chromaffin and ... More

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