Thermo Scientific™

Phire Plant Direct PCR Kit (without sampling tools)

製品番号（カタログ番号）	数量
F130WH	500 reactions

製品番号（カタログ番号） F130WH

価格（JPY）

47,200

500 reactions

一括またはカスタム形式をリクエストする

Thermo Scientific Phire植物ダイレクトPCRキットは、植物サンプルから直接DNAを増幅するために設計されています。PCRの前にDNAの精製を行なう必要はありません。このキットは、DNA結合ドメインを持つ特別に設計された酵素であるPhireホットスタートII DNAポリメラーゼをベースにしています。The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi and bacteria.

The kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA.In addition, the kit includes control primers for positive control reactions.The Control Primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• PhireホットスタートII DNAポリメラーゼ provides high yields of specific product with short extension time (20 s/kb).

Applications

• Genotyping
• Transgene detection

The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq -based polymerases.最適な結果を得るには、まず当社のTm計算機を使用して、Tmを正確に計算します。

研究用途にのみご使用ください。診断目的には使用できません。

ホットスタート内蔵ホットスタート

反応数500反応

オーバーハング平滑

ポリメラーゼPhireホットスタートII DNAポリメラーゼ

製品タイプダイレクトPCRキット

数量500 reactions

反応形態別のコンポーネント

サンプルタイプ植物

出荷条件Wet or Dry Ice

原料DNA

GC-Rich PCR Performance培地

PCR法Direct PCR：

反応速度高速

Unit SizeEach

組成および保存条件

内容：
• 200 µL PhireホットスタートII DNAポリメラーゼ
• 5 x 1.25 mL 2X Phire Plant Tissue PCR Buffer (includes dNTPs and MgCl₂)
• 40 µL Universal Control Primer mix (25 µM each)
• 2 x 5 mL Dilution Buffer

Store at -20°C.

よくあるご質問（FAQ）

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

Do the Direct PCR Master Mixes and Kits come with tissue punchers?

No, tissue punchers are not provided. You can purchase 0.3 mm Tissue Punchers (Cat. No. F-200S), or cut the sample with a scalpel. Some scientists use 100 µL pipette tips to take a sample.

View all Phire Plant Direct PCR Kit (without sampling tools) FAQs