Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire動物組織ダイレクトPCRキットは、マウス、魚、鳥および昆虫を含む、種々多様な動物組織から直接DNAを増幅するために開発されました。本キットには、動物組織から直接DNAを増幅するために必要なすべてのコンポーネントが含まれます。最適化されたPCR試薬詳細を見る

製品番号（カタログ番号）	数量
F140WH	500 reactions

製品番号（カタログ番号） F140WH

価格（JPY）

63,300

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500 reactions

一括またはカスタム形式をリクエストする

Thermo Scientific Phire動物組織ダイレクトPCRキットは、マウス、魚、鳥および昆虫を含む、種々多様な動物組織から直接DNAを増幅するために開発されました。本キットには、動物組織から直接DNAを増幅するために必要なすべてのコンポーネントが含まれます。最適化されたPCR試薬、希釈バッファー、および動物組織からのDNAの分離を強化するためのDNARealease添加剤。In addition, the kit includes control primers for positive control reactions that are universal and work with numerous animal species.A detailed manual is provided that describes protocols when working with different types of tissues.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• PhireホットスタートII DNAポリメラーゼ provides high yields of specific product with short extension time (20 s/kb).

The optimal annealing temperature for Phire DNA Polymerase may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

研究用途にのみご使用ください。診断目的には使用できません。

ホットスタート内蔵ホットスタート

反応数500反応

オーバーハング平滑

ポリメラーゼPhireホットスタートII DNAポリメラーゼ

製品タイプダイレクトPCRキット

数量500 reactions

反応形態別のコンポーネント

サンプルタイプ組織

出荷条件Wet or Dry Ice

原料DNA

GC-Rich PCR Performance培地

PCR法Direct PCR：

反応速度高速

Unit SizeEach

組成および保存条件

内容：
• 200 µL PhireホットスタートII DNAポリメラーゼ
• 5 x 1.25 mL 2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl2)
• 40 µL Control Primer mix (25 µM each)
• 2 x 5 mL Dilution Buffer
• 3 × 100 µL DNARelease Additive
• 3 × 1 mL Gel loading dye

Store at -20°C.

よくあるご質問（FAQ）

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

View all Phire Animal Tissue Direct PCR Kit (without sampling tools) FAQs