Protonation dynamics of the extracellular and cytoplasmic surface of bacteriorhodopsin in the purple membrane.
Authors:Nachliel E,Gutman M,Kiryati S,Dencher NA
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:8855251
The dynamics of proton binding to the extracellular and the cytoplasmic surfaces of the purple membrane were measured by laser-induced proton pulses. Purple membranes, selectively labeled by fluorescein at Lys-129 of bacteriorhodopsin, were pulsed by protons released in the aqueous bulk from excited pyranine (8-hydroxy-1,3,6-pyrenetrisulfonate) and the reaction of protons ... More
The engineering of membrane-permeable peptides.
Authors:Carrigan CN,Imperiali B
Journal:Analytical biochemistry
PubMed ID:15907875
Sensitive fluorescence-based thermodynamic and kinetic measurements of DNA hybridization in solution.
Authors:Morrison LE,Stols LM
Journal:Biochemistry
PubMed ID:8457571
Kinetic and thermodynamic constants associated with DNA hybridization were determined in solution using fluorescence measurements and complementary fluorophore-labeled oligomers. One oligomer was labeled with a 5'-terminal fluorescein, and the other was labeled with a 3'-terminal rhodamine. The juxtaposition of the two labels in double-stranded complexes results in a strong quenching ... More
Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction.
We have carried out fluorescence resonance energy transfer (FRET) measurements on four-way DNA junctions in order to analyze the global structure and its dependence on the concentration of several types of ions. A knowledge of the structure and its sensitivity to the solution environment is important for a full understanding ... More
Macrophage phagocytosis: use of fluorescence microscopy to distinguish between extracellular and intracellular bacteria.
Authors:Drevets DA, Campbell PA
Journal:J Immunol Methods
PubMed ID:1919019
One of the challenges of phagocytosis research is to differentiate bacteria adherent to a host cell from bacteria which the cell has internalized. To address this question, various techniques such as fluorescence microscopy, electron microscopy, and flow cytometry have been used. We have adapted a flow cytometric method (Fattorossi et ... More