Fluorescein Di-β-D-Glucopyranoside (FDGlu)
Fluorescein Di-β-D-Glucopyranoside (FDGlu)
Citations & References (5)
Invitrogen™

Fluorescein Di-β-D-Glucopyranoside (FDGlu)

フルオレセインジ diβ-D-グルコピラノシド は、グルコシダーゼの最も高感度な基質の一つです。非蛍光基質 は β-グルコシダーゼによって連続的に加水分解され、最初にフルオレセインモノグルコシド、次に高蛍光フルオレセインになります。フルオレセインジβ-D-グルコピラノシドの酵素媒介加水分解に続いて詳細を見る
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製品番号（カタログ番号）数量
F2881
または、製品番号F-2881
5 mg
製品番号（カタログ番号） F2881
または、製品番号F-2881
価格（JPY）
31,200
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Ends: 27-Mar-2026
52,100
割引額 20,900 (40%)
Each
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数量:
5 mg
フルオレセインジ diβ-D-グルコピラノシド は、グルコシダーゼの最も高感度な基質の一つです。非蛍光基質 は β-グルコシダーゼによって連続的に加水分解され、最初にフルオレセインモノグルコシド、次に高蛍光フルオレセインになります。フルオレセインジβ-D-グルコピラノシドの酵素媒介加水分解に続いて、吸光度または蛍光のいずれかが増加します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞透過性細胞透過性
励起／発光490⁄514
数量5 mg
出荷条件室温
基質β‐グルコシダーゼ基質
検出法蛍光
Substrate Properties化学基質
Unit SizeEach
組成および保存条件
冷凍庫（-5～-30℃）に保存。

引用および参考文献 (5)

引用および参考文献
Abstract
An in situ study of beta-glucosidase activity in normal and Gaucher fibroblasts with fluorogenic probes.
Authors:Kohen E, Kohen C, Hirschberg JG, Santus R, Grabowski G, Mangel W, Gatt S, Prince J
Journal:Cell Biochem Funct
PubMed ID:8403230
'Beta-glucosidase activity was evaluated in situ by means of fluorogenic probes in normal human fibroblasts and fibroblasts from homozygous carriers of the Gaucher trait. Probe internalization, targeting to lysosomes and post-cleavage probe retention were the primary concerns. Internalization and targeting were attempted by in situ photosensitized labilization of lysosomal membranes, ... More
Yeast exo-beta-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae.
Authors:Cid VJ, Alvarez AM, Santos AI, Nombela C, Sanchez M
Journal:Yeast
PubMed ID:7975893
'Yeast exo-1,3-beta-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(beta-D-glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and ... More
A flow cytometric assay for lysosomal glucocerebrosidase.
Authors:van Es HH, Veldwijk M, Havenga M, Valerio D
Journal:Anal Biochem
PubMed ID:9177687
'A flow cytometric assay is described for the determination of glucocerebrosidase (GC) activity using fluorescein di-beta-glucopyranoside (FDGlu). Fluorescent product is formed upon intracellular hydrolysis of FDGlu and is measured in the FL1 channel of a flow cytometer. We show that the assay is specific for lysosomal beta-glucosidase or glucocerebrosidase (1) ... More
Directed evolution of a thermophilic beta-glucosidase for cellulosic bioethanol production.
Authors:Hardiman E, Gibbs M, Reeves R, Bergquist P,
Journal:Appl Biochem Biotechnol
PubMed ID:19834652
Characteristics that would make enzymes more desirable for industrial applications can be improved using directed evolution. We developed a directed evolution technique called random drift mutagenesis (RNDM). Mutant populations are screened and all functional mutants are collected and put forward into the next round of mutagenesis and screening. The goal ... More
Nucleic acid detection using non-radioactive labelling methods.
Authors:Mansfield ES, Worley JM, McKenzie SE, Surrey S, Rappaport E, Fortina P
Journal:Mol Cell Probes
PubMed ID:7477006
Nucleic acid probe-based assays are now widely used in genetic research, human identification, forensics and in a broad spectrum of clinical assays in the fields of microbiology, haematology/oncology and virology. Labelled probes are used in a variety of assay formats including dot-blots, Southern blots (DNA target), Northern blots (RNA target), ... More