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Citations & References (14)
Invitrogen™
Fc OxyBURST™ Green Assay Reagent
Fc OxyBURST™アッセイ緑色試薬
| 製品番号（カタログ番号） | 数量 |
|---|---|
| F2902 | 25反応 |
製品番号（カタログ番号） F2902
価格（JPY）
88,500
Each
数量:
25反応
Fc OxyBURST™ Greenアッセイ試薬は、ジクロロジヒドロフルオレセイン（H2DCF）と共有結合され、精製ウサギポリクローナル抗BSA抗体と複合化されたウシ血清アルブミン（BSA）で構成されています。この免疫複合体がFc受容体に結合すると、非蛍光H2DCF分子が食胞内に取り込まれ、その後で緑色蛍光の2''7'-ジクロロフルオレセインに酸化されます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
濃度3 mg⁄ml
検出法蛍光
染色剤タイプフリーラディカルセンサー
形状溶液
フォーマットチューブ、スライド
数量25反応
出荷条件湿氷
溶解性DMSO（ジメチルスルホキシド）
Emission488⁄523
使用対象 (装置)蛍光顕微鏡, フローサイトメーター
製品ラインOXYBURST
製品タイプアッセイ試薬
Unit SizeEach
組成および保存条件
Fc OxyBURST™アッセイ緑色試薬（アジ化ナトリウムを含むPBS中の懸濁液）のバイアル1本が含まれます。 冷蔵庫（2～8℃）に保存し、遮光。
引用および参考文献 (14)
引用および参考文献
Abstract
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.
Journal:J Exp Med
PubMed ID:16880255
'Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA receptor were expressed
Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase.
Journal:J Immunol
PubMed ID:12594290
'The phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. Within this compartment, the TNF- and IFN-gamma-responsive acid sphingomyelinase (ASMase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin D. To investigate the possible role of ASMase as a mediator of the antibacterial
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
Journal:J Immunol Methods
PubMed ID:7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without
Fluorescent probes for living cells.
Journal:Histochem J
PubMed ID:10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion