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Citations & References (22)
Invitrogen™
FluoReporter™ lacZ/Galactosidase Quantitation Kit
FluoReporter™ lacZ/Galactosidase Quantitation Kit には、β-ガラクトシダーゼ基質、3-カルボキシイルンフェリル β-D-ガラクトピラノシドが含まれており、青色蛍光加水分解産物である 7-ヒドロキシクマリン-3詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| F2905 | 1 Kit |
製品番号（カタログ番号） F2905
価格（JPY）お問い合わせください ›
72,100
Each
数量:
1 Kit
FluoReporter™ lacZ/Galactosidase Quantitation Kit には、β-ガラクトシダーゼ基質、3-カルボキシイルンフェリル β-D-ガラクトピラノシドが含まれており、青色蛍光加水分解産物である 7-ヒドロキシクマリン-3 カルボン酸を生成します。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
アッセイβ-Gal（lacZ）アッセイ
検出法蛍光
製品ラインFluoReporter
製品タイプβ-Galactosidase Reporter Gene Assay System
数量1 Kit
出荷条件室温
基質CUG
基質特性化学基質
基質タイプβ-Gal基質
標的酵素β-ガラクトシダーゼ
フォーマットキット
Unit SizeEach
組成および保存条件
フリーザー（-5～-30度）に保存し、遮光してください。
引用および参考文献 (22)
引用および参考文献
Abstract
Substrate-labeled fluorescent immunoassay for phenobarbital.
Journal:Ther Drug Monit
PubMed ID:7013165
An assay for the anticonvulsant drug phenobarbital (PB) has been developed that is based on the principles of the substrate-labeled fluorescent immunoassay. A fluorogenic enzyme substrate, galactosyl umbelliferone, was covalently linked to a derivative of PB. The labeled drug, galactosyl umbelliferone-PB (GUPB), is nonfluorescent under conditions of the assay; however,
Substrate-labeled fluorescent immunoassay for measuring dibekacin concentrations in serum and plasma.
Journal:Antimicrob Agents Chemother
PubMed ID:6357071
'We have developed a homogeneous substrate-labeled fluorescent immunoassay for the measurement of dibekacin concentrations in serum and plasma. The fluorogenic enzyme substrate beta-galactosyl-umbelliferone was covalently attached to tobramycin, an aminoglycoside structurally similar to dibekacin, to prepare a fluorogenic drug reagent (FDR). The FDR is nonfluorescent under assay conditions, but fluoresces
A homogeneous fluorescent immunoassay for human immunoglobulin M.
Journal:Clin Chem
PubMed ID:7014030
'We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensity of the labeled IgM at 450 nm (with excitation at 400 nm) is negligible, but when
Substrate-labeled fluorescent immunoassay for phenytoin in human serum.
Journal:Clin Chem
PubMed ID:108033
'A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosidase. When antibody to
Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
Journal:J Virol
PubMed ID:12388712
'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from