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Citations & References (7)
Invitrogen™
Fluorescein Di-β-D-Glucuronide (FDGlcU)
フルオレセインジ diβ-D-グルクロニド（FDGlcU）は、モノグルクロニドに加水分解され、高蛍光フルオレセインに到達するまで、無色で非蛍光です。詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| F2915 または、製品番号F-2915 | 5 mg |
製品番号（カタログ番号） F2915
または、製品番号F-2915
価格（JPY）お問い合わせください ›
57,300
온라인 행사
Ends: 27-Mar-2026
95,500割引額 38,200 (40%)
Each
数量:
5 mg
フルオレセインジ diβ-D-グルクロニド（FDGlcU）は、モノグルクロニドに加水分解され、高蛍光フルオレセインに到達するまで、無色で非蛍光です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞透過性細胞透過性
励起／発光490⁄514
数量5 mg
出荷条件室温
基質β‐グルクロニダーゼ (GUS) 基質
検出法蛍光
Substrate Properties化学基質
Unit SizeEach
組成および保存条件
冷凍庫（-5～-30℃）に保存。
よくあるご質問（FAQ）
Can I make a higher stock solution of Fluorescein Di-β-D-Glucuronide (FDGlcU) (Cat. No. F2915) e.g., 50 mM in PBS?
What is the molecular weight of Fluorescein Di-β-D-Glucuronide (FDGlcU) (Cat. No. F2915)?
引用および参考文献 (7)
引用および参考文献
Abstract
Single cell analysis and selection of living retrovirus vector-corrected mucopolysaccharidosis VII cells using a fluorescence-activated cell sorting-based assay for mammalian beta-glucuronidase enzymatic activity.
Journal:J Biol Chem
PubMed ID:9872999
'Mutations in the acid beta-glucuronidase gene lead to systemic accumulation of undegraded glycosaminoglycans in lysosomes and ultimately to clinical manifestations of mucopolysaccharidosis VII (Sly disease). Gene transfer by retrovirus vectors into murine mucopolysaccharidosis VII hematopoietic stem cells or fibroblasts ameliorates glycosaminoglycan accumulation in some affected tissues. The efficacy of gene
Gene expression imaging by enzymatic catalysis of a fluorescent probe via membrane-anchored beta-glucuronidase.
Journal:Gene Ther
PubMed ID:17235292
'Development of nonimmunogenic and specific reporter genes to monitor gene expression in vivo is important for the optimization of gene therapy protocols. We developed a membrane-anchored form of mouse beta-glucuronidase (mbetaG) as a reporter gene to hydrolyze a nonfluorescent glucuronide probe (fluorescein di-beta-D-glucuronide, (FDGlcU) to a highly fluorescent reporter to
Enzyme-generated intracellular fluorescence for single-cell reporter gene analysis utilizing Escherichia coli beta-glucuronidase.
Journal:Cytometry
PubMed ID:8866216
'We report the development of a new fluorescence-activated cell sorter (FACS)-based reporter gene system utilizing the enzymatic activity of the E. coli beta-glucuronidase (gus) gene. When loaded with the Gus substrate fluorescein-di-beta-D-glucuronide (FDGlcu), individual mammalian cells expressing and translating gus mRNA liberate sufficient levels of intracellular fluorescein for quantitative analysis
Direct influence of S9 liver homogenate on fluorescence signals: impact on practical applications in a bacterial genotoxicity assay.
Journal:Mutat Res
PubMed ID:11719102
Assays based on the bacterial SOS-response offer the possibility of automatization of genotoxicity testing for screening of large compound libraries. While existing assays use colorimetric detection or luminescence read-out, we describe here the use of a fluorescence-based system to achieve high sensitivity of detection required for assay miniaturization. Three commonly
Critical elements of oligosaccharide acceptor substrates for the Pasteurella multocida hyaluronan synthase.
Journal:J Biol Chem
PubMed ID:16361253
Three-dimensional structures are not available for polysaccharide synthases and only minimal information on the molecular basis for catalysis is known. The Pasteurella multocida hyaluronan synthase (PmHAS) catalyzes the polymerization of the alternating beta1,3-N-acetylglucosamine-beta1,4-glucuronic acid sugar chain by the sequential addition of single monosaccharides to the non-reducing terminus. Therefore, PmHAS possesses