FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain

Citations & References (23)

Invitrogen™

FM™ 4-64FX, fixable analog of FM™ 4-64 membrane stain

親油性プローブであるFM™ 4-64FXは、水中での蛍光強度が低く、細胞膜の外葉に結合することで強く蛍光を発し、個別の細胞膜染色を実現します。結合は迅速かつ可逆的で、「FX」はこのアナログがアルデヒドベースの固定剤で固定可能であることを示します。また、活発に発火しているニューロンを特定し、活性依存性小胞サイクリングのメカニズムを調査するための優れた試薬でもあります詳細を見る

製品番号（カタログ番号）	数量
F34653	10 x 100 μg

製品番号（カタログ番号） F34653

価格（JPY）

137,000

お問い合わせください ›

10 x 100 μg

親油性プローブであるFM™ 4-64FXは、水中での蛍光強度が低く、細胞膜の外葉に結合することで強く蛍光を発し、個別の細胞膜染色を実現します。結合は迅速かつ可逆的で、「FX」はこのアナログがアルデヒドベースの固定剤で固定可能であることを示します。また、活発に発火しているニューロンを特定し、活性依存性小胞サイクリングのメカニズムを調査するための優れた試薬でもあります。

研究用にのみ使用できます。診断用には使用いただけません。

検出法蛍光

使用対象 (装置)蛍光顕微鏡

製品ラインFM

数量10 x 100 μg

出荷条件室温

標識タイプFluorescent Dye

製品タイプ親油性プローブ

SubCellular Localization細胞膜

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (23)

引用および参考文献

Abstract

Crystal structure of the complete integrin alphaVbeta3 ectodomain plus an alpha/beta transmembrane fragment.

Authors:Xiong JP, Mahalingham B, Alonso JL, Borrelli LA, Rui X, Anand S, Hyman BT, Rysiok T, Müller-Pompalla D, Goodman SL, Arnaout MA,

Journal:J Cell Biol

PubMed ID:19704023

'We determined the crystal structure of 1TM-alphaVbeta3, which represents the complete unconstrained ectodomain plus short C-terminal transmembrane stretches of the alphaV and beta3 subunits. 1TM-alphaVbeta3 is more compact and less active in solution when compared with DeltaTM-alphaVbeta3, which lacks the short C-terminal stretches. The structure reveals a bent conformation and ... More

Endocytic trafficking from the small intestinal brush border probed with FM dye.

Authors:Hansen GH, Rasmussen K, Niels-Christiansen LL, Danielsen EM,

Journal:Am J Physiol Gastrointest Liver Physiol

PubMed ID:19679822

The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little ... More

A stochastic mechanism for biofilm formation by Mycoplasma pulmonis.

Authors:Simmons WL, Bolland JR, Daubenspeck JM, Dybvig K,

Journal:J Bacteriol

PubMed ID:17142389

Bacterial biofilms are communities of bacteria that are enclosed in an extracellular matrix. Within a biofilm the bacteria are protected from antimicrobials, environmental stresses, and immune responses from the host. Biofilms are often believed to have a highly developed organization that is derived from differential regulation of the genes that ... More

Activity-induced rapid synaptic maturation mediated by presynaptic cdc42 signaling.

Authors:Shen W, Wu B, Zhang Z, Dou Y, Rao ZR, Chen YR, Duan S

Journal:Neuron

PubMed ID:16675395

Maturation of presynaptic transmitter secretion machinery is a critical step in synaptogenesis. Here we report that a brief train of presynaptic action potentials rapidly converts early nonfunctional contacts between cultured hippocampal neurons into functional synapses by enhancing presynaptic glutamate release. The enhanced release was confirmed by a marked increase in ... More

Helical disposition of proteins and lipopolysaccharide in the outer membrane of Escherichia coli.

Authors:Ghosh AS, Young KD

Journal:J Bacteriol

PubMed ID:15743937

In bacteria, several physiological processes once thought to be the products of uniformly dispersed reactions are now known to be highly asymmetric, with some exhibiting interesting geometric localizations. In particular, the cell envelope of Escherichia coli displays a form of subcellular differentiation in which peptidoglycan and outer membrane proteins at ... More

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