FM™ 1-43FX, fixable analog of FM™ 1-43 membrane stain

Citations & References (35)

Invitrogen™

FM™ 1-43FX, fixable analog of FM™ 1-43 membrane stain

FM 1-43FXメンブレンプローブは、FM 1-43メンブレンプローブアナログで、脂肪族アミンを含むように改良されています。この改良により、アルデヒドベースの固定剤（ホルムアルデヒド、グルタルアルデヒドなど）でプローブを固定できるようになりました詳細を見る

製品番号（カタログ番号）	数量
F35355	10 x 100 μg

製品番号（カタログ番号） F35355

価格（JPY）

97,300

お問い合わせください ›

10 x 100 μg

FM 1-43FXメンブレンプローブは、FM 1-43メンブレンプローブアナログで、脂肪族アミンを含むように改良されています。この改良により、アルデヒドベースの固定剤（ホルムアルデヒド、グルタルアルデヒドなど）でプローブを固定できるようになりました。FM 1-43メンブレンプローブは、活発に発火しているニューロンの特定や、活動依存性小胞サイクリングのメカニズムの調査に広く使用されています。未修正のFM 1-43メンブレンプローブは、1バイアル（T-3163）に1 mg、10バイアルに1 mgパッケージ、各バイアルに100µg（T-35356）入っています。

研究用にのみ使用できます。診断用には使用いただけません。

色オレンジ

検出法蛍光

使用対象 (装置)蛍光顕微鏡

製品ラインFM

数量10 x 100 μg

出荷条件室温

標識タイプFluorescent Dye

製品タイプFM 1-43メンブレンプローブ

SubCellular Localization細胞膜

Unit SizeEach

組成および保存条件

室温で保存し、光から保護します。

よくあるご質問（FAQ）

I want to study endosomes trafficking using FM 4-64. Will the label be retained after fixation? And can I label already-fixed cells?

No. For that you would need the FM 4-64FX version. The non-FX version will not be retained upon fixation, leading to loss of much of the stain and an increase in background. The FX version will be retained using an aldehyde-based fixative. Cells that are already fixed will be stained throughout the cell and the signal will not be localized; it is recommended to stain live cells and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?

No. For that, you would need the FM 4-64FX version. The non-FX version will be lost, leading to loss of much of the specific label and a vast increase in background labeling. The FX version will be fixed in place with formaldehyde. Cells that have been fixed already will not label correctly, so you will need to label the cells live and then fix.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The product sheet for FM 1-43 Dye states that the maximum for excitation is 510 nm and emissions is 626 nm, but the spectrum on the product page shows a peak at 470 nm and 580 nm. Can you clarify what the excitation and emission maxima is for this dye?

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (35)

引用および参考文献

Abstract

A developmental switch in neurotransmitter flux enhances synaptic efficacy by affecting AMPA receptor activation.

Authors:Renger JJ, Egles C, Liu G

Journal:Neuron

PubMed ID:11239436

'Formation of glutamatergic synapses entails development of "silent" immature contacts into mature functional synapses. To determine how this transformation occurs, we investigated the development of neurotransmission at single synapses in vitro. Maturation of presynaptic function, assayed with endocytotic markers, followed accumulation of synapsin I. During this period, synaptic transmission was ... More

Directed cell growth on protein-functionalized hydrogel surfaces.

Authors:Hynd MR, Frampton JP, Dowell-Mesfin N, Turner JN, Shain W

Journal:J Neurosci Methods

PubMed ID:17368788

'Biochemical surface modification has been used to direct cell attachment and growth on a biocompatible gel surface. Acrylamide-based hydrogels were photo-polymerized in the presence of an acroyl-streptavidin monomer to create planar, functionalized surfaces capable of binding biotin-labelled proteins. Soft protein lithography (microcontact printing) of proteins was used to transfer the ... More

CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission.

Authors:Jevsek M, Jaworski A, Polo-Parada L, Kim N, Fan J, Landmesser LT, Burden SJ

Journal:Proc Natl Acad Sci U S A

PubMed ID:16606832

'The genes encoding several synaptic proteins, including acetylcholine receptors, acetylcholinesterase, and the muscle-specific kinase, MuSK, are expressed selectively by a small number of myofiber nuclei positioned near the synaptic site. Genetic analysis of mutant mice suggests that additional genes, expressed selectively by synaptic nuclei, might encode muscle-derived retrograde signals that ... More

Constitutive sharing of recycling synaptic vesicles between presynaptic boutons.

Authors:Darcy KJ, Staras K, Collinson LM, Goda Y

Journal:Nat Neurosci

PubMed ID:16462738

'The synaptic vesicle cycle is vital for sustained neurotransmitter release. It has been assumed that functional synaptic vesicles are replenished autonomously at individual presynaptic terminals. Here we tested this assumption by using FM dyes in combination with fluorescence recovery after photobleaching and correlative light and electron microscopy in cultured rat ... More

Using FM1-43 to study neuropeptide granule dynamics and exocytosis.

Authors:Brumback AC, Lieber JL, Angleson JK, Betz WJ

Journal:Methods

PubMed ID:15183177

In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide ... More

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