FluoCells™ Prepared Slide #1 (BPAE cells with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI)
FluoCells™ Prepared Slide #1 (BPAE cells with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI)
Citations & References (2)
Invitrogen™

FluoCells™ Prepared Slide #1 (BPAE cells with MitoTracker™ Red CMXRos, Alexa Fluor™ 488 Phalloidin, and DAPI)

FluoCells™調製顕微鏡スライドには、蛍光顕微鏡観察用の固定された多標識細胞調製物が含まれています。FluoCell™調製スライド#1には、ウシ肺動脈内皮細胞（BPAEC）が含まれています。MitoTracker™ Red CMXRosを使用して生細胞中のミトコンドリアを染色し、膜電位依存的に集積しました詳細を見る
テクニカルサポートオーダーサポート
製品番号（カタログ番号）数量
F36924各1個
製品番号（カタログ番号） F36924
価格（JPY）
52,800
Each
お問い合わせください ›
数量:
各1個
FluoCells™調製顕微鏡スライドには、蛍光顕微鏡観察用の固定された多標識細胞調製物が含まれています。FluoCell™調製スライド#1には、ウシ肺動脈内皮細胞（BPAEC）が含まれています。MitoTracker™ Red CMXRosを使用して生細胞中のミトコンドリアを染色し、膜電位依存的に集積しました。固定および透過処理後、F-アクチンをAlexa Fluor™ 488ファロイジンで染色し、核を青色蛍光DNA染色DAPIで対比染色しました。

当社の顕微鏡キャリブレーション試薬の全コレクションをご覧ください ›
研究用にのみ使用できます。診断用には使用いただけません。
仕様
使用対象（アプリケーション）免疫蛍光
数量各1個
出荷条件室温
製品ラインAlexa Fluor、FluoCells、MitoTracker
タイプ調製済み顕微鏡スライド
Unit SizeEach
組成および保存条件
室温で保存し、光から保護します。

よくあるご質問（FAQ）

Do you have any slides of pre-labeled cells or tissues that I can use to demonstrate with?

Some of the most popular are the FluoCells demonstration slides, which have cells or tissue sections that are labeled with three different dyes. FluoCells 1 and 2 have cultured cells, while FluoCells 3 is a tissue section (mouse kidney). Slides with fluorescent beads are also available.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

DAPI and Hoechst dyes are quite similar to each other. Why would I choose one over the other?

DAPI is a very common blue-fluorescent dye for nuclear counterstaining and gives very bright labeling on nuclei in fixed and permeabilized cells and tissues. However, it is considered to be a semi-permeant to impermeant stain and provides inconsistent staining of live cells. Hoechst 33342 dye is cell-permeant and stains with the same binding mechanism and fluorescent color; it is preferred for live-cell imaging and is just as good as DAPI for fixed cell labeling.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (2)

引用および参考文献
Abstract
Quality assessment of confocal microscopy slide based systems: performance.
Authors:Zucker RM
Journal:Cytometry A
PubMed ID:16807897
'BACKGROUND: All fluorescence slide-based cytometry detections systems basically include the following components: (1) an excitation light source, (2) intermediate optics, and (3) a detection device consisting of a CCD camera or a PMT. The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM). METHODS: The ... More
Quality assessment of confocal microscopy slide-based systems: instability.
Authors:Zucker RM
Journal:Cytometry A
PubMed ID:16807898
'BACKGROUND: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. METHODS: A number of tests have been devised to evaluate equipment performance and instability. The following four instability ... More