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Thermo Scientific™

DyNAmo ColorFlash SYBR Green qPCR Kit

**********************Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a詳細を見る
製品番号(カタログ番号)反応数
F416XL反応2500回分
F416L
または、製品番号F-416L
500 reactions
製品番号(カタログ番号) F416XL
価格(JPY)
241,700
Each
反応数:
反応2500回分
**********************
Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of DyNAmo ColorFlash SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

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Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights

• Colored reagents minimize the risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
• Extremely fast protocols due to combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination

Applications

• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Related Products
DyNamo ColorFlash Probe qPCR Kit
DyNAmo ColorFlash SYBR Green qPCR Kit
研究用途にのみご使用ください。診断目的には使用できません。
仕様
使用対象 (装置)7000 System, 7300 System, 7500 Fast System, 7500 System, 7700 System, 7900HT Fast System, 7900HT System, BioRad CFX96™, QuantStudio™, ViiA™ 7 System, Bio-Rad CFX384 Touch™, Roche LightCycler 480, 5700 Sequence Detection System, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler
フォーマットTube
キットタイプDyNAmo ColorFlash SYBR Green qPCR Kit
反応数反応2500回分
ポリメラーゼTbr DNA polymerase
製品ラインDyNAmo Flash
製品タイプReal Time PCR SYBR Master Mix
数量2,500 reactions
サンプルタイプDNA, dsDNA
対応可能対象2500 Reactions of 20 μL, 1000 reactions of 50 μL
容量25 x 1 mL
濃度2X (qPCR Mix), 50X (Dye), 40X (Buffer)
検出法SYBR
使用対象(アプリケーション)遺伝子発現解析
GC-Rich PCR Performance
PCR法qPCR
反応速度高速
Unit SizeEach
組成および保存条件
Contains:
• 25 x 1 mL DyNAmo ColorFlash SYBR Green qPCR Mix (2X)
• 1 x 1.25 mL 50X ROX dye, 25μM
• 5 x 1 mL 40X sample buffer solution with yellow dye

Store at -20°C

よくあるご質問(FAQ)

Does the yellow dye from the Yellow Sample Buffer provided with Luminaris Color and DyNAmo Flash qPCR Master Mixes influence qPCR performance?

The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.

Do I need to use Fast plastics to run a Fast qPCR reaction?

Specialized Fast PCR plates are not required if you are using a standard qPCR machine with a fast protocol. However, Fast Block PCR Plates (AB-1900) can be purchased which are compatible with the thermal blocks on fast qPCR thermal cyclers.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.