Phalloidin Labeling Probes
Phalloidin Labeling Probes
Citations & References (459)
Invitrogen™

Phalloidin Labeling Probes

Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
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製品番号（カタログ番号）色励起波長域染色剤タイプ
F432Green496⁄516FITC(フルオレセイン)
A22281Blue346⁄442Alexa Fluor™ 350
A30104Violet405/450Alexa Fluor™ Plus 405
A12379Green495⁄518Alexa Fluor™ 488
O7466Green496⁄520Oregon Green™ 488
A22282Yellow531⁄554Alexa Fluor™ 532
R415Red-orange540⁄565TRITC(テトラメチルローダミンイソチオシアン酸塩)
A22283Orange556⁄570Alexa Fluor™ 546
A34055Orange555⁄565Alexa Fluor™ 555
A30106Orange555/565 nmAlexa Fluor Plus 555
B3475Red558⁄569BODIPY™
A12380Orange-red578⁄600Alexa Fluor™ 568
A12381Red581⁄609Alexa Fluor™ 594
T7471Red591⁄608Texas Red™
A22284Far-red632⁄647Alexa Fluor™ 633
A34054Far-red633⁄647Alexa Fluor™ 635
A22287Far-red650⁄668Alexa Fluor™ 647
A30107Far-red650/668 nmAlexa Fluor Plus 647
A22285Near-infrared663⁄690Alexa Fluor™ 660
A22286Near-infrared679⁄702Alexa Fluor™ 680
A30105Near-infrared758/784Alexa Fluor™ Plus 750
B7474NoneNoneビオチン-XX
P3457NoneNonePhalloidin (unlabeled)
製品番号（カタログ番号） F432
価格（JPY）
129,500
Each
お問い合わせください ›
色:
Green
励起波長域:
496⁄516
染色剤タイプ:
FITC(フルオレセイン)
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.

A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.

Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.

Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining

Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.

Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.

Features of phalloidin probes

  • High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
  • Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
  • Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
  • Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
  • Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
  • Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
  • Ease of use—staining is straightforward and quick
  • Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
  • Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton
研究用にのみ使用できます。診断用には使用いただけません。
仕様
色Green
染色剤タイプFITC(フルオレセイン)
励起波長域496⁄516
使用対象 (装置)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible with FITC/GFP filter set
数量300 Units
出荷条件室温
標識タイプ従来の色素
製品タイプファロイジン
SubCellular Localizationアクチン、細胞骨格, Cytoskeleton
Unit SizeEach
組成および保存条件
フリーザー(-5°C∼-30°C)に保存し、遮光してください。

よくあるご質問（FAQ）

I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?

One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用および参考文献 (459)

引用および参考文献
Abstract
The p42/p44 MAP kinase pathway prevents apoptosis induced by anchorage and serum removal.
Authors:Le Gall M,Chambard JC,Breittmayer JP,Grall D,Pouysségur J,Van Obberghen-Schilling E
Journal:Molecular biology of the cell
PubMed ID:10712523
Anchorage removal like growth factor removal induces apoptosis. In the present study we have characterized signaling pathways that can prevent this cell death using a highly growth factor– and anchorage-dependent line of lung fibroblasts (CCL39). After anchorage removal from exponentially growing cells, annexin V-FITC labeling can be detected after 8 ... More
Basilar papilla explants: a model to study hair cell regeneration-repair and protection.
Authors:Frenz DA, Yoo H, Liu W
Journal:Acta Otolaryngol
PubMed ID:9840500
Explants of basilar papillae from 6-7 days posthatch chicks were cultured in growth medium for a period of 1-8 days. Hair cells were counted following staining of stereocilia bundles with FITC-phalloidin, and the percentage of hair cell survival was determined by comparison to control (i.e. uncultured) specimens. Hair cell integrity ... More
Responses induced by tacrine in neuronal and non-neuronal cell lines.
Authors:De Ferrari GV, von Bernhardi R, Calderón FH, Luza SC, Inestrosa NC
Journal:J Neurosci Res
PubMed ID:9589388
Alzheimer's disease (AD) is associated with a reduction in cholinergic activity as a result of specific neuronal loss. Current potential treatments for the disease include both cholinomimetic drugs and anticholinesterase inhibitors. One of the drugs approved by the FDA is tacrine (9-amine-1,2,3,4 tetrahydroacridine; THA), a strong acetylcholinesterase (AChE) inhibitor. We ... More
The small GTP-binding protein Rac promotes the dissociation of gelsolin from actin filaments in neutrophils.
Authors:Arcaro A
Journal:J Biol Chem
PubMed ID:9422735
Gelsolin is an actin filament-capping protein that has been shown to play a key role in cell migration. Here we have studied the involvement of phosphoinositide 3-kinase (PI 3-kinase) and GTP-binding proteins (G-proteins) in the regulation of gelsolin-actin interactions in neutrophils. Inhibition of PI 3-kinase activity in vivo by wortmannin ... More
Integrin-mediated activation of focal adhesion kinase is independent of focal adhesion formation or integrin activation. Studies with activated and inhibitory beta3 cytoplasmic domain mutants.
Authors:Lyman S, Gilmore A, Burridge K, Gidwitz S, White GC
Journal:J Biol Chem
PubMed ID:9278407
Integrin alphaIIbbeta3 functions as the fibrinogen receptor on platelets and mediates platelet aggregation and clot retraction. Among the events that occur during either "inside-out" or "outside-in" signaling through alphaIIbbeta3 is the phosphorylation of focal adhesion kinase (pp125(FAK)) and the association of pp125(FAK) with cytoskeletal components. To examine the role of ... More
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