Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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製品番号（カタログ番号）化学物質名または材質色
F518LHF Buffer PackColorless
F519LGC Buffer PackColorless
F520LDetergent-free FF Buffer PackColorless
F521LDetergent-free GC Buffer PackColorless
F538LHF Buffer PackGreen
F539LGC Buffer PackGreen
製品番号（カタログ番号） F518L
価格（JPY）
4,400
Each
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化学物質名または材質:
HF Buffer Pack
色:
Colorless
一括またはカスタム形式をリクエストする
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
研究用途にのみご使用ください。診断目的には使用できません。
仕様
化学物質名または材質HF Buffer Pack
色Colorless
概要Phusion™ HFバッファーパック
内容Phusion HFバッファーパック（F-518L）の内容：
•5倍Phusion HFバッファー（1倍バッファーは50 mM MgCl2を提供）
•1.5 mM MgCl2
• DMSO

-20 ℃で保存します。
使用対象（アプリケーション）Standard PCR
数量6mL
Unit SizeEach