Phusion™ HF and GC Buffer Packs
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Thermo Scientific™

Phusion™ HF and GC Buffer Packs

5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
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製品番号（カタログ番号）化学物質名または材質色
F539LGC Buffer PackGreen
F518LHF Buffer PackColorless
F519LGC Buffer PackColorless
F520LDetergent-free FF Buffer PackColorless
F521LDetergent-free GC Buffer PackColorless
F538LHF Buffer PackGreen
製品番号（カタログ番号） F539L
価格（JPY）
4,000
Each
お問い合わせください ›
化学物質名または材質:
GC Buffer Pack
色:
Green
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

  • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
  • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))
研究用途にのみご使用ください。診断目的には使用できません。
仕様
化学物質名または材質GC Buffer Pack
色Green
内容PhusionグリーンGCバッファーパック（F-539L）の内容：
• 5X PhusionグリーンGCバッファー（1Xバッファーは1.5 mM MgCl2入り）
• 50 mM MgCl2
• DMSO

-20℃で保存液。
使用対象（アプリケーション）Standard PCR
数量4 x 1.5 mL
Unit SizeEach

よくあるご質問（FAQ）

Can I use Phusion Green and Phire Green PCR products for subsequent applications such as sequencing, ligation, and restriction digestion?

Yes. The dyes do not interfere with downstream applications such as DNA sequencing, ligation, and restriction digestion.

What dyes are present in Phusion Green and Phire Green buffers?

Phusion Green and Phire Green buffers contain two dyes for monitoring electrophoresis progress. The blue dye migrates with 3-5 kb DNA fragments and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have excitation peaks at 424 nm and 615 nm, respectively.