Thermo Scientific Phusion Blood Direct PCR Kitは、全血からのDNAの増幅用に開発されました。これにより、PCRの前に個別のDNA浄化ステップを行う必要がなくなります。改良型PhusionホットスタートIIハイフィデリティDNAポリメラーゼは、血液中に見られるPCR阻害剤に対して高い耐性を示し、全血の40%を含むサンプルでもポリメラーゼ活性を維持します。
このDNAポリメラーゼは、プルーフリーディングにおいて卓越した精度を示し、Phusion Blood Direct PCRキットは、クローニングなどの要求の厳しいアプリケーションに最適な選択肢の1つです。Phusion Blood Direct PCRキットは汎用性が高く、さまざまな種のさまざまな抗凝固剤や保存条件での血液サンプルの分析も可能です。 特長:
内容: • 40 µL Phusion血液II DNAポリメラーゼ • 1.25 mL 2倍Phusion血液PCRバッファー(dNTPおよびMgCl2を含む) • 40 µLユニバーサルコントロールプライマーミックス • 0.5 mL 50 mM EDTA • 1.5 mL 50 mM MgCl2 • 0.5 mL DMSO
-20 ℃にて保管してください。
よくあるご質問(FAQ)
What is your recommendation to take a sample from plant or animal tissue for Direct PCR?
In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.
Why are Control primers provided in the Direct PCR Kits?
It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.
Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?
You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.
Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?
Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.
What is the highest blood concentration possible in the reaction when using Phusion Blood Direct PCR Master Mix and Kit?
We recommend using blood concentration up to 20%; however, we had successful amplifications with up to 40% of blood in the reaction mix. In some cases optimization may be required to achieve this.